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Cloning
This category includes novel approaches for generating and joining DNA fragments. DNA fragments may be produced chemically, from RNA templates or from DNA with specific attributes (genome location, repetitive nature, viral, polymorphic) Procedures can be specific for subcloning or for more large scale projects such as whole genome or cDNA libraries. (see also GENOMICS). Variations might include subtracted or normalized libraries. New vectors may be presented only if based on novel principles worthy of own publication on their own.
Citations 1-10 of 64 total displayed.
- Methods Online
An efficient method to assemble linear DNA templates for in vitro screening and selection systems
- Viktor Stein and Florian Hollfelder
Nucleic Acids Res. 2009; 37: e122.
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- Methods Online
Novel method for high-throughput colony PCR screening in nanoliter-reactors
- Marcel Walser, Rene Pellaux, Andreas Meyer, Matthias Bechtold, Herve Vanderschuren, Richard Reinhardt, Joseph Magyar, Sven Panke, and Martin Held
Nucleic Acids Res. 2009; 37: e57.
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- Methods Online
A sequence-independent in vitro transposon-based strategy for efficient cloning of genomes of large DNA viruses as bacterial artificial chromosomes
- Fuchun Zhou, Qiuhua Li, and Shou-Jiang Gao
Nucleic Acids Res. 2009; 37: e2.
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- Methods Online
Rapid and reliable universal cloning of influenza A virus genes by target-primed plasmid amplification
- Jürgen Stech, Olga Stech, Astrid Herwig, Hermann Altmeppen, Jana Hundt, Sandra Gohrbandt, Anne Kreibich, Siegfried Weber, Hans-Dieter Klenk, and Thomas C. Mettenleiter
Nucleic Acids Res. 2008; 36: e139.
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- Methods Online
De novo DNA synthesis using single molecule PCR
- Tuval Ben Yehezkel, Gregory Linshiz, Hen Buaron, Shai Kaplan, Uri Shabi, and Ehud Shapiro
Nucleic Acids Res. 2008; 36: e107.
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- Methods Online
Rapid one-step recombinational cloning
- Changlin Fu, Daniel R. Wehr, Janice Edwards, and Brian Hauge
Nucleic Acids Res. 2008; 36: e54.
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- Methods Online
Predicting RNA-binding sites from the protein structure based on electrostatics, evolution and geometry
- Yao Chi Chen and Carmay Lim
Nucleic Acids Res. 2008; 36: e29.
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- Methods Online
Microplate-based chromatin immunoprecipitation method, Matrix ChIP: a platform to study signaling of complex genomic events
- Steve Flanagin, Joel D. Nelson, David G. Castner, Oleg Denisenko, and Karol Bomsztyk
Nucleic Acids Res. 2008; 36: e17.
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- Methods Online
One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning
- Al-Muataz Khalil, Jeffrey A. Julius, and Jeff Bachant
Nucleic Acids Res. 2007; 35: e104.
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- Methods Online
Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry
- Eva Kühn-Hölsken, Olexandr Dybkov, Björn Sander, Reinhard Lührmann, and Henning Urlaub
Nucleic Acids Res. 2007; 35: e95.
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