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Cloning
This category includes novel approaches for generating and joining DNA fragments. DNA fragments may be produced chemically, from RNA templates or from DNA with specific attributes (genome location, repetitive nature, viral, polymorphic) Procedures can be specific for subcloning or for more large scale projects such as whole genome or cDNA libraries. (see also GENOMICS). Variations might include subtracted or normalized libraries. New vectors may be presented only if based on novel principles worthy of own publication on their own.
Citations 11-20 of 65 total displayed.
- Methods Online
Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry
- Eva Kühn-Hölsken, Olexandr Dybkov, Björn Sander, Reinhard Lührmann, and Henning Urlaub
Nucleic Acids Res. 2007; 35: e95.
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- Methods Online
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
- Fernando Geu-Flores, Hussam H. Nour-Eldin, Morten T. Nielsen, and Barbara A. Halkier
Nucleic Acids Res. 2007; 35: e55.
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- Methods Online
Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs
- Hirohide Saito, Tamiko Minamisawa, and Kiyotaka Shiba
Nucleic Acids Res. 2007; 35: e38.
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- Methods Online
A versatile ligation-independent cloning method suitable for high-throughput expression screening applications
- Nick S. Berrow, David Alderton, Sarah Sainsbury, Joanne Nettleship, Rene Assenberg, Nahid Rahman, David I. Stuart, and Raymond J. Owens
Nucleic Acids Res. 2007; 35: e45.
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- Nucleic Acid Enzymes
USERTM friendly DNA engineering and cloning method by uracil excision
- Jurate Bitinaite, Michelle Rubino, Kamini Hingorani Varma, Ira Schildkraut, Romualdas Vaisvila, and Rita Vaiskunaite
Nucleic Acids Res. 2007; 35: 1992-2002.
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- Methods Online
A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus
- Tiziana Aucelli, Patrizia Contursi, Michele Girfoglio, Mosè Rossi, and Raffaele Cannio
Nucleic Acids Res. 2006; 34: e114.
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- Methods Online
Mouse microRNA profiles determined with a new and sensitive cloning method
- Shuji Takada, Eugene Berezikov, Yoshihiro Yamashita, Mariana Lagos-Quintana, Wigard P. Kloosterman, Munehiro Enomoto, Hisashi Hatanaka, Shin-ichiro Fujiwara, Hideki Watanabe, Manabu Soda, Young Lim Choi, Ronald H. A. Plasterk, Edwin Cuppen, and Hiroyuki Mano
Nucleic Acids Res. 2006; 34: e115.
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- Methods Online
Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
- Hussam H. Nour-Eldin, Bjarne G. Hansen, Morten H. H. Nørholm, Jacob K. Jensen, and Barbara A. Halkier
Nucleic Acids Res. 2006; 34: e122.
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- Methods Online
A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus
- Tiziana Aucelli, Patrizia Contursi, Michele Girfoglio, Mosè Rossi, and Raffaele Cannio
Nucleic Acids Res. Advance Access published on September 13, 2006; doi: 10.1093/nar/gkl615.
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- Methods Online
mazF, a novel counter-selectable marker for unmarked chromosomal manipulation in Bacillus subtilis
- Xiao-Zhou Zhang, Xin Yan, Zhong-Li Cui, Qing Hong, and Shun-Peng Li
Nucleic Acids Res. 2006; 34: e71.
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