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Cloning
This category includes novel approaches for generating and joining DNA fragments. DNA fragments may be produced chemically, from RNA templates or from DNA with specific attributes (genome location, repetitive nature, viral, polymorphic) Procedures can be specific for subcloning or for more large scale projects such as whole genome or cDNA libraries. (see also GENOMICS). Variations might include subtracted or normalized libraries. New vectors may be presented only if based on novel principles worthy of own publication on their own.
Citations 21-30 of 64 total displayed.
- Methods Online
Rapid and efficient cDNA library screening by self-ligation of inverse PCR products (SLIP)
- Roger A. Hoskins, Mark Stapleton, Reed A. George, Charles Yu, Kenneth H. Wan, Joseph W. Carlson, and Susan E. Celniker
Nucleic Acids Res. 2005; 33: e185.
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- Methods Online
Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library
- Sonia Schoonbroodt, Nicolas Frans, Mark DeSouza, Rachel Eren, Smadar Priel, Naama Brosh, Judith Ben-Porath, Arie Zauberman, Ehud Ilan, Shlomo Dagan, Edward H. Cohen, Hennie R. Hoogenboom, Robert Charles Ladner, and René M. Hoet
Nucleic Acids Res. 2005; 33: e81.
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- Methods Online
Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage
- Markus Fuhrmann, Wolfgang Oertel, Peter Berthold, and Peter Hegemann
Nucleic Acids Res. 2005; 33: e58.
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- Methods Online
A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo
- Olli H. Laitinen, Kari J. Airenne, Vesa P. Hytönen, Erik Peltomaa, Anssi J. Mähönen, Thomas Wirth, Miia M. Lind, Kari A. Mäkelä, Pyry I. Toivanen, Diana Schenkwein, Tommi Heikura, Henri R. Nordlund, Markku S. Kulomaa, and Seppo Ylä-Herttuala
Nucleic Acids Res. 2005; 33: e42.
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- Methods Online
Simple and highly efficient BAC recombineering using galK selection
- Søren Warming, Nina Costantino, Donald L. Court, Nancy A. Jenkins, and Neal G. Copeland
Nucleic Acids Res. 2005; 33: e36.
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- Methods Online
Transposon-mediated generation of targeting vectors for the production of gene knockouts
- Chunfang Zhang, Danny Kitsberg, Hun Chy, Qi Zhou, and John R. Morrison
Nucleic Acids Res. 2005; 33: e24.
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- Methods Online
Evaluation of vector-primed cDNA library production from microgram quantities of total RNA
- Jonathan Kuo, Jason Inman, Michael Brownstein, and Ted B. Usdin
Nucleic Acids Res. 2004; 32: e183.
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- Methods Online
Induction and characterization of taxol-resistance phenotypes with a transiently expressed artificial transcriptional activator library
- Dong-ki Lee, Yong Ha Kim, Jin-Soo Kim, and Wongi Seol
Nucleic Acids Res. 2004; 32: e116.
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- Methods Online
A simple, rapid, high-fidelity and cost-effective PCR-based two-step DNA synthesis method for long gene sequences
- Ai-Sheng Xiong, Quan-Hong Yao, Ri-He Peng, Xian Li, Hui-Qin Fan, Zong-Ming Cheng, and Yi Li
Nucleic Acids Res. 2004; 32: e98.
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- NAR Methods Online
Two-step total gene synthesis method
- Lei Young and Qihan Dong
Nucleic Acids Res. 2004; 32: e59.
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