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Cloning
This category includes novel approaches for generating and joining DNA fragments. DNA fragments may be produced chemically, from RNA templates or from DNA with specific attributes (genome location, repetitive nature, viral, polymorphic) Procedures can be specific for subcloning or for more large scale projects such as whole genome or cDNA libraries. (see also GENOMICS). Variations might include subtracted or normalized libraries. New vectors may be presented only if based on novel principles worthy of own publication on their own.
Citations 31-40 of 65 total displayed.
- NAR Methods Online
Two-step total gene synthesis method
- Lei Young and Qihan Dong
Nucleic Acids Res. 2004; 32: e59.
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- NAR Methods Online
Simple cDNA normalization using kamchatka crab duplex-specific nuclease
- Pavel A. Zhulidov, Ekaterina A. Bogdanova, Alex S. Shcheglov, Laura L. Vagner, George L. Khaspekov, Valery B. Kozhemyako, Mikhail V. Matz, Ella Meleshkevitch, Leonid L. Moroz, Sergey A. Lukyanov, and Dmitry A. Shagin
Nucleic Acids Res. 2004; 32: e37.
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- NAR Methods Online
Oligonucleotide-directed site-specific integration of high complexity libraries into ssDNA templates
- M. B. Hale, G. P. Nolan, and R. Wolkowicz
Nucleic Acids Res. 2004; 32: e22.
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- NAR Methods Online
Construction of long DNA molecules using long PCR-based fusion of several fragments simultaneously
- Nikolai A. Shevchuk, Anton V. Bryksin, Yevgeniya A. Nusinovich, Felipe C. Cabello, Margaret Sutherland, and Stephan Ladisch
Nucleic Acids Res. 2004; 32: e19.
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- NAR Methods Online
Improved full-length cDNA production based on RNA tagging by T4 DNA ligase
- Christian Clepet, Isabelle Le Clainche, and Michel Caboche
Nucleic Acids Res. 2004; 32: e6.
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- NAR Methods Online
One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid
- Kenji Tsuge, Kuniko Matsui, and Mitsuhiro Itaya
Nucleic Acids Res. 2003; 31: e133.
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- NAR Methods Online
Identification of a novel proliferation-inducing determinant using lentiviral expression cloning
- Dmitri Chilov, Cornelia Fux, Hana Joch, and Martin Fussenegger
Nucleic Acids Res. 2003; 31: e113.
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- NAR Methods Online
Optimum conditions for selective isolation of genes from complex genomes by transformation-associated recombination cloning
- Sun-Hee Leem, Vladimir N. Noskov, Jung-Eun Park, Seung Il Kim, Vladimir Larionov, and Natalay Kouprina
Nucleic Acids Res. 2003; 31: e29.
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- Articles
Efficient cloning and engineering of entire mitochondrial genomes in Escherichia coli and transfer into transcriptionally active mitochondria
- Young Geol Yoon and Michael D. Koob
Nucleic Acids Res. 2003; 31: 1407-1415.
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- NAR Methods Online
Random DNA fragmentation with endonuclease V: application to DNA shuffling
- Kentaro Miyazaki
Nucleic Acids Res. 2002; 30: e139.
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