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Cloning
This category includes novel approaches for generating and joining DNA fragments. DNA fragments may be produced chemically, from RNA templates or from DNA with specific attributes (genome location, repetitive nature, viral, polymorphic) Procedures can be specific for subcloning or for more large scale projects such as whole genome or cDNA libraries. (see also GENOMICS). Variations might include subtracted or normalized libraries. New vectors may be presented only if based on novel principles worthy of own publication on their own.
Citations 41-50 of 64 total displayed.
- NAR Methods Online
BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting
- Mark L. Crowe, Debashis Rana, Fiona Fraser, Ian Bancroft, and Martin Trick
Nucleic Acids Res. 2002; 30: e118.
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- NAR Methods Online
Rapid gene cloning using terminator primers and modular vectors
- William F. Donahue, Brian M. Turczyk, and Kevin A. Jarrell
Nucleic Acids Res. 2002; 30: e95.
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- NAR Methods Online
A reliable and efficient method for deleting operational sequences in PACs and BACs
- Ravi Nistala and Curt D. Sigmund
Nucleic Acids Res. 2002; 30: e41.
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- NAR Methods Online
A two-step strategy for constructing specifically self-subtracted cDNA libraries
- Paolo Laveder, Cristiano De Pittà, Stefano Toppo, Giorgio Valle, and Gerolamo Lanfranchi
Nucleic Acids Res. 2002; 30: e38.
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- NAR Methods Online
A novel strategy for the functional cloning of enzymes using filamentous phage display: the case of nucleotidyl transferases
- Erika Brunet, Camille Chauvin, Valérie Choumet, and Jean-Luc Jestin
Nucleic Acids Res. 2002; 30: e40.
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- Articles
Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by ‘reconditioning PCR’
- Janelle R. Thompson, Luisa A. Marcelino, and Martin F. Polz
Nucleic Acids Res. 2002; 30: 2083-2088.
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- NAR Methods Online
A new approach to genome mapping and sequencing: slalom libraries
- Veronika I. Zabarovska, Rinat Z. Gizatullin, Ali N. Al-Amin, Raf Podowski, Alexei I. Protopopov, Sven Löfdahl, Claes Wahlestedt, Gösta Winberg, Vladimir I. Kashuba, Ingemar Ernberg, and Eugene R. Zabarovsky
Nucleic Acids Res. 2002; 30: e6.
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- NAR Methods Online
A genetic system for direct selection of gene-positive clones during recombinational cloning in yeast
- Vladimir Noskov, Natalay Kouprina, Sun-Hee Leem, Maxim Koriabine, J. Carl Barrett, and Vladimir Larionov
Nucleic Acids Res. 2002; 30: e8.
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- NAR Methods Online
Defining the minimal length of sequence homology required for selective gene isolation by TAR cloning
- V. N. Noskov, M. Koriabine, G. Solomon, M. Randolph, J. C. Barrett, S.-H. Leem, L. Stubbs, N. Kouprina, and V. Larionov
Nucleic Acids Res. 2001; 29: e32.
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- NAR Methods Online
Escherichia coli strains that allow antibiotic-free plasmid selection and maintenance by repressor titration
- Rocky M. Cranenburgh, Julian A. J. Hanak, Steven G. Williams, and David J. Sherratt
Nucleic Acids Res. 2001; 29: e26.
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