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Cloning
This category includes novel approaches for generating and joining DNA fragments. DNA fragments may be produced chemically, from RNA templates or from DNA with specific attributes (genome location, repetitive nature, viral, polymorphic) Procedures can be specific for subcloning or for more large scale projects such as whole genome or cDNA libraries. (see also GENOMICS). Variations might include subtracted or normalized libraries. New vectors may be presented only if based on novel principles worthy of own publication on their own.
Citations 51-60 of 64 total displayed.
- NAR Methods Online
Directional cDNA library construction assisted by the in vitro recombination reaction
- Osamu Ohara and Gary Temple
Nucleic Acids Res. 2001; 29: e22.
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- NAR Methods Online
Isolation of segments of homologous genes with only one conserved amino acid region via PCR
- Martin Laging, Berthold Fartmann, and Wilfried Kramer
Nucleic Acids Res. 2001; 29: e8.
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- NAR Methods Online
Non-gridded library: a new approach for BAC (bacterial artificial chromosome) exploitation in hexaploid wheat (Triticum aestivum)
- Zhiying Ma, Song Weining, Peter J. Sharp, and Chunji Liu
Nucleic Acids Res. 2000; 28: e106.
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- NAR Methods Online
Mirror orientation selection (MOS): a method for eliminating false positive clones from libraries generated by suppression subtractive hybridization
- Denis V. Rebrikov, Olga V. Britanova, Nadya G. Gurskaya, Konstantin A. Lukyanov, Victor S. Tarabykin, and Sergey A. Lukyanov
Nucleic Acids Res. 2000; 28: e90.
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- Articles
Functional analysis of putative restriction–modification system genes in the Helicobacter pylori J99 genome
- Huimin Kong, Lee-Fong Lin, Nicole Porter, Shawn Stickel, Devon Byrd, Janos Posfai, and Richard J. Roberts
Nucleic Acids Res. 2000; 28: 3216-3223.
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- NAR Methods Online
A novel method for constructing gene-targeting vectors
- Kiyotaka Akiyama, Hirotaka Watanabe, Shuichi Tsukada, and Hitoshi Sasai
Nucleic Acids Res. 2000; 28: e77.
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- Articles
The DNase activity of RNase T and its application to DNA cloning
- Y Zuo and MP Deutscher
Nucleic Acids Res. 1999; 27: 4077-4082.
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- NAR Methods Online
Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites
- D Tillett and BA Neilan
Nucleic Acids Res. 1999; 27: e26.
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- NAR Methods Online
Use of a linear multicopy vector based on the mini-replicon of temperate coliphage N15 for cloning DNA with abnormal secondary structures
- NV Ravin and VK Ravin
Nucleic Acids Res. 1999; 27: e13.
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- NAR Methods Online
Simplified generation of targeting constructs using ET recombination
- PO Angrand, N Daigle, F van der Hoeven, HR Scholer, and AF Stewart
Nucleic Acids Res. 1999; 27: e16.
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