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Cloning
This category includes novel approaches for generating and joining DNA fragments. DNA fragments may be produced chemically, from RNA templates or from DNA with specific attributes (genome location, repetitive nature, viral, polymorphic) Procedures can be specific for subcloning or for more large scale projects such as whole genome or cDNA libraries. (see also GENOMICS). Variations might include subtracted or normalized libraries. New vectors may be presented only if based on novel principles worthy of own publication on their own.
Citations 61-64 of 64 total displayed.
- Articles
Rapid modification of bacterial artificial chromosomes by ET- recombination
- JP Muyrers, Y Zhang, G Testa, and AF Stewart
Nucleic Acids Res. 1999; 27: 1555-1557.
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- Articles
A new signal sequence trap using alkaline phosphatase as a reporter
- H Chen and P Leder
Nucleic Acids Res. 1999; 27: 1219-1222.
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- Articles
Substantially enhanced cloning efficiency of SAGE (Serial Analysis of Gene Expression) by adding a heating step to the original protocol
- M Kenzelmann and K Muhlemann
Nucleic Acids Res. 1999; 27: 917-918.
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- Articles
Rapid and efficient cloning of proviral flanking fragments by kanamycin resistance gene complementation
- B Fehse, K K hlcke, A Langer, W Ostertag, and H Lother
Nucleic Acids Res. 1999; 27: 706-707.
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