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DNA characterisation


This category includes all new methods, or highly significant improvement thereof, for the biochemical and physical characterization of DNA. This includes DNA purification from various sources (e.g. ancient DNA, DNA from widely used hosts), DNA quantification, DNA analysis (by enzymatic, gel electrophoresis, mass-spectrometry, hybridization, assay) as well as determination of DNA sequences.


Citations 11-20 of 178 total displayed.

Past content

Methods Online
Chemical mapping of cytosines enzymatically flipped out of the DNA helix
Dalia Daujotyte, Zita Liutkeviciute, Gintautas Tamulaitis, and Saulius Klimasauskas
Nucleic Acids Res. 2008; 36: e57. [Abstract] [Full text] [Print PDF]  

Molecular Biology
LINE-1 methylation status of endogenous DNA double-strand breaks
Wichai Pornthanakasem, Narisorn Kongruttanachok, Chutipa Phuangphairoj, Chotika Suyarnsestakorn, Taweap Sanghangthum, Sornjarod Oonsiri, Wanpen Ponyeam, Thatchawan Thanasupawat, Oranart Matangkasombut, and Apiwat Mutirangura
Nucleic Acids Res. 2008; 36: 3667-3675. [Abstract] [Full text] [Print PDF]  

Methods Online
Improved genome-wide localization by ChIP-chip using double-round T7 RNA polymerase-based amplification
Harm van Bakel, Folkert J. van Werven, Marijana Radonjic, Mariel O. Brok, Dik van Leenen, Frank C. P. Holstege, and H. T. Marc Timmers
Nucleic Acids Res. 2008; 36: e21. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Genomics
Genome-wide tracking of unmethylated DNA Alu repeats in normal and cancer cells
Jairo Rodriguez, Laura Vives, Mireia Jordà, Cristina Morales, Mar Muñoz, Elisenda Vendrell, and Miguel A. Peinado
Nucleic Acids Res. 2008; 36: 770-784. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing
Matthias Meyer, Adrian W. Briggs, Tomislav Maricic, Barbara Höber, Barbara Höffner, Johannes Krause, Antje Weihmann, Svante Pääbo, and Michael Hofreiter
Nucleic Acids Res. 2008; 36: e5. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A ligation assay for multiplex analysis of CpG methylation using bisulfite-treated DNA
Christina Dahl and Per Guldberg
Nucleic Acids Res. 2007; 35: e144. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Kinetic studies of Escherichia coli AlkB using a new fluorescence-based assay for DNA demethylation
Todd W. Roy and A. S. Bhagwat
Nucleic Acids Res. 2007; 35: e147. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Chemistry
DNA multiplex hybridization on microarrays and thermodynamic stability in solution: a direct comparison
Daniel J. Fish, M. Todd Horne, Greg P. Brewood, Jim P. Goodarzi, Saba Alemayehu, Ashwini Bhandiwad, Robert P. Searles, and Albert S. Benight
Nucleic Acids Res. 2007; 35: 7197-7208. [Abstract] [Full text] [Print PDF]  

Methods Online
A pyrosequencing-tailored nucleotide barcode design unveils opportunities for large-scale sample multiplexing
Poornima Parameswaran, Roxana Jalili, Li Tao, Shadi Shokralla, Baback Gharizadeh, Mostafa Ronaghi, and Andrew Z. Fire
Nucleic Acids Res. 2007; 35: e130. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Molecular Biology
Novel high-resolution characterization of ancient DNA reveals C > U-type base modification events as the sole cause of post mortem miscoding lesions
Paul Brotherton, Phillip Endicott, Juan J. Sanchez, Mark Beaumont, Ross Barnett, Jeremy Austin, and Alan Cooper
Nucleic Acids Res. 2007; 35: 5717-5728. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

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