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DNA transfer
This category describes novel methods for introducing and maintaining DNA in cells and in transgenic animals. This may involve selection markers, integrating versus extrachromosomal vectors, and mechanisms for increasing efficiencies of transformation/transfection.
Citations 1-10 of 63 total displayed.
- Synthetic Biology and Chemistry
Oligopeptide-mediated gene transfer into mouse corneal endothelial cells: expression, design optimization, uptake mechanism and nuclear localization
- Wei Yang Seow, Yi-Yan Yang, and Andrew J. T. George
Nucleic Acids Res. 2009; 37: 6276-6289.
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- Methods Online
Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells
- Omar Nyabi, Michael Naessens, Katharina Haigh, Agnieszka Gembarska, Steven Goossens, Marion Maetens, Sarah De Clercq, Benjamin Drogat, Lieven Haenebalcke, Sonia Bartunkova, Ilse De Vos, Bram De Craene, Mansour Karimi, Geert Berx, Andras Nagy, Pierre Hilson, Jean-Christophe Marine, and Jody J. Haigh
Nucleic Acids Res. 2009; 37: e55.
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- Methods Online
Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
- Gaurav Backliwal, Markus Hildinger, Sebastien Chenuet, Sarah Wulhfard, Maria De Jesus, and Florian M. Wurm
Nucleic Acids Res. 2008; 36: e96.
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- Methods Online
Conditional gene vectors regulated in cis
- Dagmar Pich, Sibille Humme, Mark-Peter Spindler, Aloys Schepers, and Wolfgang Hammerschmidt
Nucleic Acids Res. 2008; 36: e83.
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- Methods Online
Nuclear delivery of NF B-assisted DNA/polymer complexes: plasmid DNA quantitation by confocal laser scanning microscopy and evidence of nuclear polyplexes by FRET imaging
- Gilles Breuzard, Magdalena Tertil, Cristine Gonçalves, Hervé Cheradame, Philippe Géguan, Chantal Pichon, and Patrick Midoux
Nucleic Acids Res. 2008; 36: e71.
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- Methods Online
Terminal proteins of Streptomyces chromosome can target DNA into eukaryotic nuclei
- Hsiu-Hui Tsai, Chih-Hung Huang, Alan M. Lin, and Carton W. Chen
Nucleic Acids Res. 2008; 36: e62.
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- Molecular Biology
Intracellular delivery of an anionic antisense oligonucleotide via receptor-mediated endocytosis
- Md Rowshon Alam, Vidula Dixit, Hyunmin Kang, Zi-Bo Li, Xiaoyuan Chen, JoAnn Trejo, Michael Fisher, and Rudy L. Juliano
Nucleic Acids Res. 2008; 36: 2764-2776.
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- Methods Online
Ultrasound-mediated DNA transfer for bacteria
- Yizhi Song, Thomas Hahn, Ian P. Thompson, Timothy J. Mason, Gail M. Preston, Guanghe Li, Larysa Paniwnyk, and Wei E. Huang
Nucleic Acids Res. 2007; 35: e129.
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- Methods Online
An S/MAR-based infectious episomal genomic DNA expression vector provides long-term regulated functional complementation of LDLR deficiency
- Michele M.P. Lufino, Roberto Manservigi, and Richard Wade-Martins
Nucleic Acids Res. 2007; 35: e98.
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- Methods Online
Nuclear accumulation of plasmid DNA can be enhanced by non-selective gating of the nuclear pore
- Roosmarijn E. Vandenbroucke, Bart Lucas, Joseph Demeester, Stefaan C. De Smedt, and Niek N. Sanders
Nucleic Acids Res. 2007; 35: e86.
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