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DNA transfer


This category describes novel methods for introducing and maintaining DNA in cells and in transgenic animals. This may involve selection markers, integrating versus extrachromosomal vectors, and mechanisms for increasing efficiencies of transformation/transfection.


Citations 11-20 of 63 total displayed.

Past content

Methods Online
Generation of an inducible and optimized piggyBac transposon system
Juan Cadiñanos and Allan Bradley
Nucleic Acids Res. 2007; 35: e87. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Small multicopy, non-integrative shuttle vectors based on the plasmid pRN1 for Sulfolobus acidocaldarius and Sulfolobus solfataricus, model organisms of the (cren-)archaea
Silvia Berkner, Dennis Grogan, Sonja-Verena Albers, and Georg Lipps
Nucleic Acids Res. 2007; 35: e88. [Abstract] [Full text] [Print PDF]  

Methods Online
A tractable method for simultaneous modifications to the head and tail of bacteriophage lambda and its application to enhancing phage-mediated gene delivery
Christine N. Zanghi, Ramil Sapinoro, Birgit Bradel-Tretheway, and Stephen Dewhurst
Nucleic Acids Res. 2007; 35: e59. [Abstract] [Full text] [Print PDF]  

Methods Online
A non-covalent peptide-based carrier for in vivo delivery of DNA mimics
May C. Morris, Edwige Gros, Gudrun Aldrian-Herrada, Michael Choob, John Archdeacon, Frederic Heitz, and Gilles Divita
Nucleic Acids Res. 2007; 35: e49. [Abstract] [Full text] [Print PDF]  

Methods Online
Transduction of human embryonic stem cells by ecotropic retroviral vectors
Philipp Koch, Henrike Siemen, Andrea Biegler, Joseph Itskovitz-Eldor, and Oliver Brüstle
Nucleic Acids Res. 2006; 34: e120. [Abstract] [Full text] [Print PDF]  

Methods Online
Production of transmitochondrial cybrids containing naturally occurring pathogenic mtDNA variants
Deborah Pye, Dimitra S. Kyriakouli, Geoffrey A. Taylor, Riem Johnson, Matthias Elstner, Brigitte Meunier, Zofia M. A. Chrzanowska-Lightowlers, Robert W. Taylor, Douglass M. Turnbull, and Robert N. Lightowlers
Nucleic Acids Res. 2006; 34: e95. [Abstract] [Full text] [Print PDF] [ Supplementary data ]  

Methods Online
A novel and simple method for construction of recombinant adenoviruses
Rong Tan, Chunhua Li, Sijing Jiang, and Lixin Ma
Nucleic Acids Res. 2006; 34: e89. [Abstract] [Full text] [Print PDF]  

Methods Online
Iterative in vivo assembly of large and complex transgenes by combining the activities of {varphi}C31 integrase and Cre recombinase
Felix Dafhnis-Calas, Zhengyao Xu, Steve Haines, Sunir K. Malla, Margaret C. M. Smith, and William R. A. Brown
Nucleic Acids Res. 2005; 33: e189. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
In vitro non-viral gene delivery with nanofibrous scaffolds
Dehai Liang, Yen K. Luu, Kwangsok Kim, Benjamin S. Hsiao, Michael Hadjiargyrou, and Benjamin Chu
Nucleic Acids Res. 2005; 33: e170. [Abstract] [Full text] [Print PDF]  

Methods Online
Transformation of isolated mammalian mitochondria by bacterial conjugation
Young Geol Yoon and Michael D. Koob
Nucleic Acids Res. 2005; 33: e139. [Abstract] [Full text] [Print PDF]  

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