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DNA transfer


This category describes novel methods for introducing and maintaining DNA in cells and in transgenic animals. This may involve selection markers, integrating versus extrachromosomal vectors, and mechanisms for increasing efficiencies of transformation/transfection.


Citations 31-40 of 63 total displayed.

Past content

Methods Online
Negatively charged self-assembling DNA/poloxamine nanospheres for in vivo gene transfer
Bruno Pitard, Mahajoub Bello-Roufaï, Olivier Lambert, Peggy Richard, Léa Desigaux, Sarah Fernandes, Caroline Lanctin, Hélène Pollard, Mehdi Zeghal, Pierre-Yves Rescan, and Denis Escande
Nucleic Acids Res. 2004; 32: e159. [Abstract] [Full text] [Print PDF]  

Methods Online
Pattern of genes influenced by conditional expression of the transcription factors HNF6, HNF4{alpha} and HNF1ß in a pancreatic ß-cell line
Heike Thomas, Sabine Senkel, Silke Erdmann, Tanja Arndt, Gülüzar Turan, Ludger Klein-Hitpass, and Gerhart U. Ryffel
Nucleic Acids Res. 2004; 32: e150. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
Acinetobacter sp. ADP1: an ideal model organism for genetic analysis and genome engineering
David Metzgar, Jamie M. Bacher, Valérie Pezo, John Reader, Volker Döring, Paul Schimmel, Philippe Marlière, and Valérie de Crécy-Lagard
Nucleic Acids Res. 2004; 32: 5780-5790. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Enhanced transfection efficiency of a systemically delivered tumor-targeting immunolipoplex by inclusion of a pH-sensitive histidylated oligolysine peptide
Wei Yu, Kathleen F. Pirollo, Bin Yu, Antonina Rait, Laiman Xiang, Weiqun Huang, Qi Zhou, Gözen Ertem, and Esther H. Chang
Nucleic Acids Res. 2004; 32: e48. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Surfection: a new platform for transfected cell arrays
Fu-Hsiung Chang, Chien-Hsin Lee, Ming-Ta Chen, Chun-Chen Kuo, Yi-Lin Chiang, Chi-Ying Hang, and Steve Roffler
Nucleic Acids Res. 2004; 32: e33. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
DNA modification and functional delivery into human cells using Escherichia coli DH10B
Kumaran Narayanan and Peter E. Warburton
Nucleic Acids Res. 2003; 31: e51. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Bypassing antibiotic selection: positive screening of genetically modified cells with an antigen-dependent proliferation switch
Masahiro Kawahara, Hiroshi Ueda, Sumiyo Morita, Kouhei Tsumoto, Izumi Kumagai, and Teruyuki Nagamune
Nucleic Acids Res. 2003; 31: e32. [Abstract] [Full text] [Print PDF]  

Articles
Efficient cloning and engineering of entire mitochondrial genomes in Escherichia coli and transfer into transcriptionally active mitochondria
Young Geol Yoon and Michael D. Koob
Nucleic Acids Res. 2003; 31: 1407-1415. [Abstract] [Full text] [Print PDF]  

Articles
Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3
Barbara Schrammeijer, Amke den Dulk-Ras, Annette C. Vergunst, Esmeralda Jurado Jácome, and Paul J. J. Hooykaas
Nucleic Acids Res. 2003; 31: 860-868. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Promoter-trapping in Saccharomyces cerevisiae by radiation-assisted fragment insertion
Markus Kiechle, Palaniyandi Manivasakam, Friederike Eckardt-Schupp, Robert H. Schiestl, and Anna A. Friedl
Nucleic Acids Res. 2002; 30: e136. [Abstract] [Full text] [Print PDF]  

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