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DNA transfer


This category describes novel methods for introducing and maintaining DNA in cells and in transgenic animals. This may involve selection markers, integrating versus extrachromosomal vectors, and mechanisms for increasing efficiencies of transformation/transfection.


Citations 51-60 of 63 total displayed.

Past content

NAR Methods Online
A rapid genetic screening system for identifying gene-specific suppression constructs for use in human cells
Greg M. Arndt, Margaret Patrikakis, and David Atkins
Nucleic Acids Res. 2000; 28: e15. [Abstract] [Full text] [Print PDF]  

Articles
Determination of L1 retrotransposition kinetics in cultured cells
Eric M. Ostertag, Eline T. Luning Prak, Ralph J. DeBerardinis, John V. Moran, and Haig H. Kazazian, Jr
Nucleic Acids Res. 2000; 28: 1418-1423. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A simplified method of generating transgenic Xenopus
Duncan B. Sparrow, Branko Latinkic, and Tim J. Mohun
Nucleic Acids Res. 2000; 28: e12. [Abstract] [Full text] [Print PDF]  

Articles
Histidylated oligolysines increase the transmembrane passage and the biological activity of antisense oligonucleotides
Chantal Pichon, Mahajoub Bello Roufaï, Michel Monsigny, and Patrick Midoux
Nucleic Acids Res. 2000; 28: 504-512. [Abstract] [Full text] [Print PDF]  

Articles
Plasmid DNA size does not affect the physicochemical properties of lipoplexes but modulates gene transfer efficiency
P Kreiss, B Cameron, R Rangara, P Mailhe, O Aguerre-Charriol, M Airiau, D Scherman, J Crouzet, and B Pitard
Nucleic Acids Res. 1999; 27: 3792-3798. [Abstract] [Full text] [Print PDF]  

Articles
The stability of DNA triplexes inside cells as studied by iodine-125 radioprinting
OA Sedelnikova, IG Panyutin, AN Luu, and RD Neumann
Nucleic Acids Res. 1999; 27: 3844-3850. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Targeted deletions created in yeast vectors by recombinational excision
M Duno, C Bendixen, L Krejci, and B Thomsen
Nucleic Acids Res. 1999; 27: e1. [Abstract] [Full text]  

Articles
Long-term stability of large insert genomic DNA episomal shuttle vectors in human cells
R Wade-Martins, J Frampton, and MR James
Nucleic Acids Res. 1999; 27: 1674-1682. [Abstract] [Full text] [Print PDF]  

Articles
An improved method for routine preparation of intact artificial chromosome DNA (340-1000 kb) for transfection into human cells
ST Compton, KA Henning, M Chen, MK Mansoura, and MA Ashlock
Nucleic Acids Res. 1999; 27: 1762-1765. [Abstract] [Full text] [Print PDF]  

Articles
Use of an internal ribosome entry site for bicistronic expression of Cre recombinase or rtTA transactivator
J Lindeberg and T Ebendal
Nucleic Acids Res. 1999; 27: 1552-1554. [Abstract] [Full text] [Print PDF]  

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