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Enzyme assays
This category presents methods for characterizing enzymes involved in nucleic acid biochemistry, not limited to nucleases, polymerases, methylases, phosphatases, ligases, recombinases and synthases or nucleic acids with inherent enzymatic activity.
Citations 1-10 of 50 total displayed.
- Methods Online
A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
- Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res. 2009; 37: e128.
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- Gene Regulation, Chromatin and Epigenetics
Analysis of individual remodeled nucleosomes reveals decreased histone–DNA contacts created by hSWI/SNF
- Karim Bouazoune, Tina B. Miranda, Peter A. Jones, and Robert E. Kingston
Nucleic Acids Res. 2009; 37: 5279-5294.
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- Methods Online
Plasmid-based lacZ assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
- Stanislaw K. Jozwiakowski and Bernard A. Connolly
Nucleic Acids Res. 2009; 37: e102.
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- Methods Online
A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU)
- Siripan Limsirichaikul, Atsuko Niimi, Heather Fawcett, Alan Lehmann, Shunichi Yamashita, and Tomoo Ogi
Nucleic Acids Res. 2009; 37: e31.
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- Methods Online
Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction
- Qiuping Guo, Xiaohai Yang, Kemin Wang, Weihong Tan, Wei Li, Hongxing Tang, and Huimin Li
Nucleic Acids Res. 2009; 37: e20.
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- Methods Online
A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding
- Carina Frauer and Heinrich Leonhardt
Nucleic Acids Res. 2009; 37: e22.
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- Nucleic Acid Enzymes
Functional and structural characterization of the integrase from the prototype foamy virus
- Eugene Valkov, Saumya Shree Gupta, Stephen Hare, Anna Helander, Pietro Roversi, Myra McClure, and Peter Cherepanov
Nucleic Acids Res. 2009; 37: 243-255.
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- Methods Online
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature
- Haukur Gudnason, Martin Dufva, D.D. Bang, and Anders Wolff
Nucleic Acids Res. 2007; 35: e127.
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- Methods Online
Back to basics: the untreated rabbit reticulocyte lysate as a competitive system to recapitulate cap/poly(A) synergy and the selective advantage of IRES-driven translation
- Ricardo Soto Rifo, Emiliano P. Ricci, Didier Décimo, Olivier Moncorgé, and Théophile Ohlmann
Nucleic Acids Res. 2007; 35: e121.
[Abstract]
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- Methods Online
Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry
- Kenji Fukui, Yoshio Takahata, Noriko Nakagawa, Seiki Kuramitsu, and Ryoji Masui
Nucleic Acids Res. 2007; 35: e100.
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