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Enzyme assays


This category presents methods for characterizing enzymes involved in nucleic acid biochemistry, not limited to nucleases, polymerases, methylases, phosphatases, ligases, recombinases and synthases or nucleic acids with inherent enzymatic activity.


Citations 1-10 of 50 total displayed.

Most recent content

Methods Online
A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases
Dorjbal Dorjsuren, David M. Wilson, III, William A. Beard, John P. McDonald, Christopher P. Austin, Roger Woodgate, Samuel H. Wilson, and Anton Simeonov
Nucleic Acids Res. 2009; 37: e128. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Past content

Gene Regulation, Chromatin and Epigenetics
Analysis of individual remodeled nucleosomes reveals decreased histone–DNA contacts created by hSWI/SNF
Karim Bouazoune, Tina B. Miranda, Peter A. Jones, and Robert E. Kingston
Nucleic Acids Res. 2009; 37: 5279-5294. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Plasmid-based lacZ{alpha} assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase
Stanislaw K. Jozwiakowski and Bernard A. Connolly
Nucleic Acids Res. 2009; 37: e102. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A rapid non-radioactive technique for measurement of repair synthesis in primary human fibroblasts by incorporation of ethynyl deoxyuridine (EdU)
Siripan Limsirichaikul, Atsuko Niimi, Heather Fawcett, Alan Lehmann, Shunichi Yamashita, and Tomoo Ogi
Nucleic Acids Res. 2009; 37: e31. [Abstract] [Full text] [Print PDF]  

Methods Online
Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction
Qiuping Guo, Xiaohai Yang, Kemin Wang, Weihong Tan, Wei Li, Hongxing Tang, and Huimin Li
Nucleic Acids Res. 2009; 37: e20. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A versatile non-radioactive assay for DNA methyltransferase activity and DNA binding
Carina Frauer and Heinrich Leonhardt
Nucleic Acids Res. 2009; 37: e22. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Nucleic Acid Enzymes
Functional and structural characterization of the integrase from the prototype foamy virus
Eugene Valkov, Saumya Shree Gupta, Stephen Hare, Anna Helander, Pietro Roversi, Myra McClure, and Peter Cherepanov
Nucleic Acids Res. 2009; 37: 243-255. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature
Haukur Gudnason, Martin Dufva, D.D. Bang, and Anders Wolff
Nucleic Acids Res. 2007; 35: e127. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Back to basics: the untreated rabbit reticulocyte lysate as a competitive system to recapitulate cap/poly(A) synergy and the selective advantage of IRES-driven translation
Ricardo Soto Rifo, Emiliano P. Ricci, Didier Décimo, Olivier Moncorgé, and Théophile Ohlmann
Nucleic Acids Res. 2007; 35: e121. [Abstract] [Full text] [Print PDF]  

Methods Online
Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry
Kenji Fukui, Yoshio Takahata, Noriko Nakagawa, Seiki Kuramitsu, and Ryoji Masui
Nucleic Acids Res. 2007; 35: e100. [Abstract] [Full text] [Print PDF]  

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