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Enzyme assays


This category presents methods for characterizing enzymes involved in nucleic acid biochemistry, not limited to nucleases, polymerases, methylases, phosphatases, ligases, recombinases and synthases or nucleic acids with inherent enzymatic activity.


Citations 21-30 of 50 total displayed.

Past content

Methods Online
Real-time monitoring of enzymatic DNA hydrolysis by electrospray ionization mass spectrometry
Robert H. H. van den Heuvel, Sara Gato, Cees Versluis, Pascal Gerbaux, Colin Kleanthous, and Albert J. R. Heck
Nucleic Acids Res. 2005; 33: e96. [Abstract] [Full text] [Print PDF]  

Methods Online
Surface plasmon field-enhanced fluorescence spectroscopy studies of primer extension reactions
Gudrun Stengel and Wolfgang Knoll
Nucleic Acids Res. 2005; 33: e69. [Abstract] [Full text] [Print PDF]  

Methods Online
Use of a restriction enzyme-digested PCR product as substrate for helicase assays
Jae-Ho Shin, John N. Reeve, and Zvi Kelman
Nucleic Acids Res. 2005; 33: e8. [Abstract] [Full text] [Print PDF]  

Articles
Polyadenylation-dependent screening assay for respiratory syncytial virus RNA transcriptase activity and identification of an inhibitor
Stephen W. Mason, Carol Lawetz, Yvon Gaudette, Florence Dô, Erika Scouten, Lisette Lagacé, Bruno Simoneau, and Michel Liuzzi
Nucleic Acids Res. 2004; 32: 4758-4767. [Abstract] [Full text] [Print PDF]  

Articles
Design and synthesis of fluorescent substrates for human tyrosyl-DNA phosphodiesterase I
Marc C. Rideout, Amy C. Raymond, and Alex B. Burgin, Jr
Nucleic Acids Res. 2004; 32: 4657-4664. [Abstract] [Full text] [Print PDF]  

Methods Online
In vitro selection of restriction endonucleases by in vitro compartmentalization
Nobuhide Doi, Shin Kumadaki, Yuko Oishi, Nobutaka Matsumura, and Hiroshi Yanagawa
Nucleic Acids Res. 2004; 32: e95. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A high-throughput assay for Tn5 Tnp-induced DNA cleavage
Brandon Ason and William S. Reznikoff
Nucleic Acids Res. 2004; 32: e83. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Ligase-mediated construction of branched DNA strands: a novel DNA joining activity catalyzed by T4 DNA ligase
Maritha Mendel-Hartvig, Anil Kumar, and Ulf Landegren
Nucleic Acids Res. 2004; 32: e2. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons
Zhiwen Tang, Kemin Wang, Weihong Tan, Jun Li, Lingfeng Liu, Qiuping Guo, Xiangxian Meng, Changbei Ma, and Shasheng Huang
Nucleic Acids Res. 2003; 31: e148. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A real-time DNase assay (ReDA) based on PicoGreen® fluorescence
Gökhan Tolun and Richard S. Myers
Nucleic Acids Res. 2003; 31: e111. [Abstract] [Full text] [Print PDF]  

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