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Enzyme assays


This category presents methods for characterizing enzymes involved in nucleic acid biochemistry, not limited to nucleases, polymerases, methylases, phosphatases, ligases, recombinases and synthases or nucleic acids with inherent enzymatic activity.

Citations 31-40 of 50 total displayed.

Past content

NAR Methods Online
A rapid biosensor chip assay for measuring of telomerase activity using surface plasmon resonance
Chihaya Maesawa, Toru Inaba, Hidetoshi Sato, Sin Iijima, Kaoru Ishida, Masanori Terashima, Ryo Sato, Michihiro Suzuki, Akiko Yashima, Satoshi Ogasawara, Hiroki Oikawa, Nobuhiro Sato, Kazuyoshi Saito, and Tomoyuki Masuda
Nucleic Acids Res. 2003; 31: e4. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
An in vivo selection system for homing endonuclease activity
Mathias Gruen, Kathy Chang, Irina Serbanescu, and David R. Liu
Nucleic Acids Res. 2002; 30: e29. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
SYBR Green real-time telomeric repeat amplification protocol for the rapid quantification of telomerase activity
Henning Wege, Michael S. Chui, Hai T. Le, Julie M. Tran, and Mark A. Zern
Nucleic Acids Res. 2002; 31: e3. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
Characterization of a multi-functional metal-mediated nuclease by MALDI-TOF mass spectrometry
Uraiwan Puapaiboon, Jaran Jai-nhuknan, and J. A. Cowan
Nucleic Acids Res. 2001; 29: 3652-3656. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Real-time observation of a single DNA digestion by {lambda} exonuclease under a fluorescence microscope field
Shun-ichi Matsuura, Jun Komatsu, Ken Hirano, Hachiro Yasuda, Kazunori Takashima, Shinji Katsura, and Akira Mizuno
Nucleic Acids Res. 2001; 29: e79. [Abstract] [Full text] [Print PDF]  

Articles
A sensitive, single-tube assay to measure the enzymatic activities of influenza RNA polymerase and other poly(A) polymerases: application to kinetic and inhibitor analysis
Lisa Hooker, Rebecca Strong, Ralph Adams, Balraj Handa, John H. Merrett, Joseph A. Martin, and Klaus Klumpp
Nucleic Acids Res. 2001; 29: 2691-2698. [Abstract] [Full text] [Print PDF]  

Articles
A novel fluorometric oligonucleotide assay to measure O 6-methylguanine DNA methyltransferase, methylpurine DNA glycosylase, 8-oxoguanine DNA glycosylase and abasic endonuclease activities: DNA repair status in human breast carcinoma cells overexpressing methylpurine DNA glycosylase
Emiko L. Kreklau, Melissa Limp-Foster, Naili Liu, Yi Xu, Mark R. Kelley, and Leonard C. Erickson
Nucleic Acids Res. 2001; 29: 2558-2566. [Abstract] [Full text] [Print PDF]  

Articles
Examination of the DNA substrate selectivity of DNA cytosine methyltransferases using mass tagging
Valaiporn Rusmintratip, Arthur D. Riggs, and Lawrence C. Sowers
Nucleic Acids Res. 2000; 28: 3594-3599. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA
Jianwei Jeffery Li, Ron Geyer, and Weihong Tan
Nucleic Acids Res. 2000; 28: e52. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
AFM characterization of single strand-specific endonuclease activity on linear DNA
Kazuo Umemura, Fuji Nagami, Takao Okada, and Reiko Kuroda
Nucleic Acids Res. 2000; 28: e39. [Abstract] [Full text] [Print PDF]  

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