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Genomics


This category presents new strategies for the genomic scale analyses. Examples include, but are not limited to, positional or whole genome cloning (e.g. using HAC, BAC, YAC, or P1 systems, see also CLONING), mapping, large scale sequencing, synteny characterization and the global evaluation of genes and their expression patterns (see also MONITORING GENE EXPRESSION). Strategies involve traditional molecular biology methods as well as new technologies such as arrays.


Citations 201-210 of 260 total displayed.

Past content

NAR Methods Online
NotI passporting to identify species composition of complex microbial systems
Veronika Zabarovska, Alexey S. Kutsenko, Lev Petrenko, Gelena Kilosanidze, Olle Ljungqvist, Elisabeth Norin, Tore Midtvedt, Gösta Winberg, Roland Möllby, Vladimir I. Kashuba, Ingemar Ernberg, and Eugene R. Zabarovsky
Nucleic Acids Res. 2003; 31: e5. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Sequence tagged microsatellite profiling (STMP): improved isolation of DNA sequence flanking target SSRs
M. J. Hayden, G. Good, and P. J. Sharp
Nucleic Acids Res. 2002; 30: e129. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
SNP genotyping on a genome-wide amplified DOP-PCR template
Struan F. A. Grant, Simone Steinlicht, Ulrike Nentwich, Rainer Kern, Barbara Burwinkel, and Ralf Tolle
Nucleic Acids Res. 2002; 30: e125. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
BACFinder: genomic localisation of large insert genomic clones based on restriction fingerprinting
Mark L. Crowe, Debashis Rana, Fiona Fraser, Ian Bancroft, and Martin Trick
Nucleic Acids Res. 2002; 30: e118. [Abstract] [Full text] [Print PDF]  

Articles
Engineered viruses to select genes encoding secreted and membrane-bound proteins in mammalian cells
Pierre Moffatt, Patrick Salois, Marie-Hélène Gaumond, Natalie St-Amant, Éric Godin, and Christian Lanctôt
Nucleic Acids Res. 2002; 30: 4285-4294. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Single nucleotide polymorphism genotyping using short, fluorescently labeled locked nucleic acid (LNA) probes and fluorescence polarization detection
Anton Simeonov and Theo T. Nikiforov
Nucleic Acids Res. 2002; 30: e91. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Genome-wide comparison of differences in the integration sites of interspersed repeats between closely related genomes
Ilgar Mamedov, Anastasia Batrak, Anton Buzdin, Elena Arzumanyan, Yuri Lebedev, and Eugene D. Sverdlov
Nucleic Acids Res. 2002; 30: e71. [Abstract] [Full text] [Print PDF]  

Articles
Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae
Jane A. Thanassi, Sandra L. Hartman-Neumann, Thomas J. Dougherty, Brian A. Dougherty, and Michael J. Pucci
Nucleic Acids Res. 2002; 30: 3152-3162. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
An efficient strategy for large-scale high-throughput transposon-mediated sequencing of cDNA clones
Yaron S. N. Butterfield, Marco A. Marra, Jennifer K. Asano, Susanna Y. Chan, Ranabir Guin, Martin I. Krzywinski, Soo Sen Lee, Kim W. K. MacDonald, Carrie A. Mathewson, Teika E. Olson, Pawan K. Pandoh, Anna-Liisa Prabhu, Angelique Schnerch, Ursula Skalska, Duane E. Smailus, Jeff M. Stott, Miranda I. Tsai, George S. Yang, Scott D. Zuyderduyn, Jacqueline E. Schein, and Steven J. M. Jones
Nucleic Acids Res. 2002; 30: 2460-2468. [Abstract] [Full text] [Print PDF]  

Articles
Systematic sequencing of cDNA clones using the transposon Tn5
Yuriy Shevchenko, Gerard G. Bouffard, Yaron S. N. Butterfield, Robert W. Blakesley, James L. Hartley, Alice C. Young, Marco A. Marra, Steven J. M. Jones, Jeffrey W. Touchman, and Eric D. Green
Nucleic Acids Res. 2002; 30: 2469-2477. [Abstract] [Full text] [Print PDF]  

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