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Monitoring gene expression


This category incorporates new techniques for detecting expression of specific genes or for following the simultaneous expression of many genes. Methods allowing the identification of new genes based on their expression patterns are also presented. Significant changes to standard methods (hybridization, cDNA libraries, subtracted hybridization) as well as new global strategies (RDA, DDRT-PCR, SAGE, arrays) are presented.


Citations 1-10 of 134 total displayed.

Most recent content

Computational Biology
Internal standard-based analysis of microarray data. Part 1: analysis of differential gene expressions
Igor Dozmorov and Ivan Lefkovits
Nucleic Acids Res. 2009; 37: 6323-6339. [Abstract] [Full text] [Print PDF]  

Past content

Methods Online
Conserved principles of mammalian transcriptional regulation revealed by RNA half-life
Caroline C. Friedel, Lars Dölken, Zsolt Ruzsics, Ulrich H. Koszinowski, and Ralf Zimmer
Nucleic Acids Res. 2009; 37: e115. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Imaging individual microRNAs in single mammalian cells in situ
Jing Lu and Andrew Tsourkas
Nucleic Acids Res. 2009; 37: e100. [Abstract] [Full text] [Print PDF]  

Methods Online
Labeled microRNA pull-down assay system: an experimental approach for high-throughput identification of microRNA-target mRNAs
Ren-Jun Hsu, Hsin-Jung Yang, and Huai-Jen Tsai
Nucleic Acids Res. 2009; 37: e77. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Computational Biology
Allegro: Analyzing expression and sequence in concert to discover regulatory programs
Yonit Halperin, Chaim Linhart, Igor Ulitsky, and Ron Shamir
Nucleic Acids Res. 2009; 37: 1566-1579. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Deep sequencing-based expression analysis shows major advances in robustness, resolution and inter-lab portability over five microarray platforms
Peter A. C. 't Hoen, Yavuz Ariyurek, Helene H. Thygesen, Erno Vreugdenhil, Rolf H. A. M. Vossen, Renée X. de Menezes, Judith M. Boer, Gert-Jan B. van Ommen, and Johan T. den Dunnen
Nucleic Acids Res. 2008; 36: e141. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
Pieter Mestdagh, Tom Feys, Nathalie Bernard, Simone Guenther, Caifu Chen, Frank Speleman, and Jo Vandesompele
Nucleic Acids Res. 2008; 36: e143. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Gene Regulation, Chromatin and Epigenetics
Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish
Leighcraft A. Shakes, Tennison L. Malcolm, Kevin L. Allen, Supriyo De, Ken R. Harewood, and Pradeep K. Chatterjee
Nucleic Acids Res. 2008; 36: 6237-6248. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Highly sensitive and specific microRNA expression profiling using BeadArray technology
Jing Chen, Jean Lozach, Eliza Wickham Garcia, Bret Barnes, Shujun Luo, Ivan Mikoulitch, Lixin Zhou, Gary Schroth, and Jian-Bing Fan
Nucleic Acids Res. 2008; 36: e87. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Light activation of transcription: photocaging of nucleotides for control over RNA polymerization
André Vidal Pinheiro, Pedro Baptista, and João Carlos Lima
Nucleic Acids Res. 2008; 36: e90. [Abstract] [Full text] [Print PDF]  

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