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Monitoring gene expression


This category incorporates new techniques for detecting expression of specific genes or for following the simultaneous expression of many genes. Methods allowing the identification of new genes based on their expression patterns are also presented. Significant changes to standard methods (hybridization, cDNA libraries, subtracted hybridization) as well as new global strategies (RDA, DDRT-PCR, SAGE, arrays) are presented.

Citations 101-110 of 134 total displayed.

Past content

NAR Methods Online
Gene expression profiling using a novel method: amplified differential gene expression (ADGE)
Zhijian J. Chen, Hongxie Shen, and Kenneth D. Tew
Nucleic Acids Res. 2001; 29: e46. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Optimizing the detection of nascent transcripts by RNA fluorescence in situ hybridization
Catherine D. van Raamsdonk and Shirley M. Tilghman
Nucleic Acids Res. 2001; 29: e42. [Abstract] [Full text] [Print PDF]  

Articles
Development of an inducible pol III transcription system essentially requiring a mutated form of the TATA-binding protein
Wolfgang Meissner, Heike Rothfels, Beatrix Schäfer, and Klaus Seifart
Nucleic Acids Res. 2001; 29: 1672-1682. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A GFP-equipped bidirectional expression module well suited for monitoring tetracycline-regulated gene expression in mouse
H. E. Krestel, M. Mayford, P. H. Seeburg, and R. Sprengel
Nucleic Acids Res. 2001; 29: e39. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
RNA: a method to specifically inhibit PCR amplification of known members of a multigene family by degenerate primers
Peter S. T. Yuen, Kimberly M. Brooks, and Yang Li
Nucleic Acids Res. 2001; 29: e31. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Streptogramin- and tetracycline-responsive dual regulated expression of p27Kip1 sense and antisense enables positive and negative growth control of Chinese hamster ovary cells
Cornelia Fux, Samuel Moser, Stefan Schlatter, Markus Rimann, James E. Bailey, and Martin Fussenegger
Nucleic Acids Res. 2001; 29: e19. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Development of a sensitive multi-well colorimetric assay for active NF{kappa}B
Patricia Renard, Isabelle Ernest, Andrée Houbion, Muriel Art, Hervé Le Calvez, Martine Raes, and José Remacle
Nucleic Acids Res. 2001; 29: e21. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Production by quantitative photolithographic synthesis of individually quality checked DNA microarrays
Markus Beier and Jörg D. Hoheisel
Nucleic Acids Res. 2000; 28: e11. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions
Joanne D. Andreadis and Linda A. Chrisey
Nucleic Acids Res. 2000; 28: e5. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
RET: a poly A-trap retrovirus vector for reversible disruption and expression monitoring of genes in living cells
Y Ishida and P Leder
Nucleic Acids Res. 1999; 27: e35. [Abstract] [Full text] [Print PDF]  

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