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Monitoring gene expression


This category incorporates new techniques for detecting expression of specific genes or for following the simultaneous expression of many genes. Methods allowing the identification of new genes based on their expression patterns are also presented. Significant changes to standard methods (hybridization, cDNA libraries, subtracted hybridization) as well as new global strategies (RDA, DDRT-PCR, SAGE, arrays) are presented.


Citations 71-80 of 134 total displayed.

Past content

Methods Online
Increasing the efficiency of SAGE adaptor ligation bydirected ligation chemistry
Austin P. So, Robin F. B. Turner, and Charles A. Haynes
Nucleic Acids Res. 2004; 32: e96. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Real-time measurement of in vitro transcription
Salvatore A. E. Marras, Benjamin Gold, Fred Russell Kramer, Issar Smith, and Sanjay Tyagi
Nucleic Acids Res. 2004; 32: e72. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

NAR Methods Online
Dual FRET molecular beacons for mRNA detection in living cells
Philip J. Santangelo, Brent Nix, Andrew Tsourkas, and Gang Bao
Nucleic Acids Res. 2004; 32: e57. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Peptide-linked molecular beacons for efficient delivery and rapid mRNA detection in living cells
Nitin Nitin, Philip J. Santangelo, Gloria Kim, Shuming Nie, and Gang Bao
Nucleic Acids Res. 2004; 32: e58. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A high-throughput method to monitor the expression of microRNA precursors
Thomas D. Schmittgen, Jinmai Jiang, Qian Liu, and Liuqing Yang
Nucleic Acids Res. 2004; 32: e43. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

NAR Methods Online
Expression profiling via novel multiplex assay allows rapid assessment of gene regulation in defined signalling pathways
Eric Eldering, C. Arnold Spek, Hella L. Aberson, Annette Grummels, Ingrid A. Derks, Alex F. de Vos, Cathal J. McElgunn, and Jan P. Schouten
Nucleic Acids Res. 2003; 31: e153. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
Simultaneous gene expression analysis of steady-state and actively translated mRNA populations from osteosarcoma MG-63 cells in response to IL-1{alpha} via an open expression analysis platform
Jingfang Ju, Chunli Huang, Stacey A. Minskoff, Jane E. Mayotte, Bruce E. Taillon, and Jan F. Simons
Nucleic Acids Res. 2003; 31: 5157-5166. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Streptomyces-derived quorum-sensing systems engineered for adjustable transgene expression in mammalian cells and mice
Wilfried Weber, Ronald Schoenmakers, Manuela Spielmann, Marie Daoud El-Baba, Marc Folcher, Bettina Keller, Cornelia C. Weber, Nils Link, Petra van de Wetering, Christoph Heinzen, Benoît Jolivet, Urs Séquin, Dominique Aubel, Charles J. Thompson, and Martin Fussenegger
Nucleic Acids Res. 2003; 31: e71. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

NAR Methods Online
Design of artificial transcription factors to selectively regulate the pro-apoptotic bax gene
D. Falke, M. Fisher, D. Ye, and R. L. Juliano
Nucleic Acids Res. 2003; 31: e10. [Abstract] [Full text] [Print PDF]  

Articles
Functional studies of the PI(3)-kinase signalling pathway employing synthetic and expressed siRNA
Frank Czauderna, Melanie Fechtner, Hüseyin Aygün, Wolfgang Arnold, Anke Klippel, Klaus Giese, and Jörg Kaufmann
Nucleic Acids Res. 2003; 31: 670-682. [Abstract] [Full text] [Print PDF]  

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