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Monitoring gene expression


This category incorporates new techniques for detecting expression of specific genes or for following the simultaneous expression of many genes. Methods allowing the identification of new genes based on their expression patterns are also presented. Significant changes to standard methods (hybridization, cDNA libraries, subtracted hybridization) as well as new global strategies (RDA, DDRT-PCR, SAGE, arrays) are presented.


Citations 81-90 of 134 total displayed.

Past content

Articles
Translational fusions with the engrailed repressor domain efficiently convert plant transcription factors into dominant-negative functions
Heike Markel, John Chandler, and Wolfgang Werr
Nucleic Acids Res. 2002; 30: 4709-4719. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Improved quantitative real-time RT–PCR for expression profiling of individual cells
Birgit Liss
Nucleic Acids Res. 2002; 30: e89. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Detection of promoter activity by flow cytometric analysis of GFP reporter expression
Anne-Lyse Ducrest, Mario Amacker, Joachim Lingner, and Markus Nabholz
Nucleic Acids Res. 2002; 30: e65. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Detection of oligonucleotide hybridization at femtomolar level and sequence-specific gene analysis of the Arabidopsis thaliana leaf extract with an ultrasensitive surface plasmon resonance spectrometer
Fayi Song, Feimeng Zhou, Jun Wang, Nongjian Tao, Jianqiao Lin, Robert L. Vellanoweth, Yvonne Morquecho, and Janel Wheeler-Laidman
Nucleic Acids Res. 2002; 30: e72. [Abstract] [Full text] [Print PDF]  

Articles
Dominant genetic screen for cofactors that enhance antisense RNA-mediated gene silencing in fission yeast
Mitch Raponi and Greg M. Arndt
Nucleic Acids Res. 2002; 30: 2546-2554. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

NAR Methods Online
Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
Michael W. Pfaffl, Graham W. Horgan, and Leo Dempfle
Nucleic Acids Res. 2002; 30: e36. [Abstract] [Full text] [Print PDF]  

Articles
Post-transcriptional suppression of gene expression in Xenopus embryos by small interfering RNA
Yuan Zhou, Yick-Pang Ching, Kin Hang Kok, Hsiang-fu Kung, and Dong-Yan Jin
Nucleic Acids Res. 2002; 30: 1664-1669. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A novel medium throughput quantitative competitive PCR technology to simultaneously measure mRNA levels from multiple genes
Junlong Zhang, Ian N. M. Day, and Christopher D. Byrne
Nucleic Acids Res. 2002; 30: e20. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Statistical evaluation of differential expression on cDNA nylon arrays with replicated experiments
Ralf Herwig, Pia Aanstad, Matthew Clark, and Hans Lehrach
Nucleic Acids Res. 2001; 29: e117. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Signal amplification by rolling circle amplification on DNA microarrays
Girish Nallur, Chenghua Luo, Linhua Fang, Stephanie Cooley, Varshal Dave, Jeremy Lambert, Kari Kukanskis, Stephen Kingsmore, Roger Lasken, and Barry Schweitzer
Nucleic Acids Res. 2001; 29: e118. [Abstract] [Full text] [Print PDF]  

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