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Mutagenesis


This category presents widely useful methods for random or site-specific, chemical or enzymatic, in vitro or in vivo mutagenesis.


Citations 1-10 of 73 total displayed.

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Methods Online
Protein evolution by hypermutation and selection in the B cell line DT40
Hiroshi Arakawa, Hiroaki Kudo, Vera Batrak, Randolph B. Caldwell, Michael A. Rieger, Joachim W. Ellwart, and Jean-Marie Buerstedde
Nucleic Acids Res. 2008; 36: e1. [Abstract] [Full text] [Print PDF]  

Methods Online
Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli
Joseph A. DeVito
Nucleic Acids Res. 2008; 36: e4. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Past content

Methods Online
Cre reconstitution allows for DNA recombination selectively in dual-marker-expressing cells in transgenic mice
Yanwen Xu, Gang Xu, Bindong Liu, and Guoqiang Gu
Nucleic Acids Res. 2007; 35: e126. [Abstract] [Full text] [Print PDF]  

Methods Online
Site-directed transposon integration in human cells
Stephen R. Yant, Yong Huang, Bassel Akache, and Mark A. Kay
Nucleic Acids Res. 2007; 35: e50. [Abstract] [Full text] [Print PDF]  

Methods Online
Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs
Hirohide Saito, Tamiko Minamisawa, and Kiyotaka Shiba
Nucleic Acids Res. 2007; 35: e38. [Abstract] [Full text] [Print PDF]  

Methods Online
Generation of a mouse mutant by oligonucleotide-mediated gene modification in ES cells
Marieke Aarts, Marleen Dekker, Sandra de Vries, Anja van der Wal, and Hein te Riele
Nucleic Acids Res. 2006; 34: e147. [Abstract] [Full text] [Print PDF]  

Methods Online
Development of a novel site-specific mutagenesis assay using MALDI-ToF MS (SSMA-MS)
Keith I. E. McLuckie, John H. Lamb, Jatinderpal K. Sandhu, Helen L. Pearson, Karen Brown, Peter B. Farmer, and Donald J. L. Jones
Nucleic Acids Res. 2006; 34: e150. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Antibody binding loop insertions as diversity elements
Csaba Kiss, Hugh Fisher, Emanuele Pesavento, Minghua Dai, Rosa Valero, Milan Ovecka, Rhiannon Nolan, M. Lisa Phipps, Nileena Velappan, Leslie Chasteen, Jennifer S. Martinez, Geoffrey S. Waldo, Peter Pavlik, and Andrew R.M. Bradbury
Nucleic Acids Res. 2006; 34: e132. [Abstract] [Full text] [Print PDF]  

Methods Online
Mutagenesis of diploid mammalian genes by gene entrapment
Qing Lin, Sarah L. Donahue, Tracy Moore-Jarrett, Shang Cao, Anna B. Osipovich, and H. Earl Ruley
Nucleic Acids Res. 2006; 34: e139. [Abstract] [Full text] [Print PDF]  

Methods Online
Tapping diversity lost in transformations—in vitro amplification of ligation reactions
Daniel Christ, Kristoffer Famm, and Greg Winter
Nucleic Acids Res. 2006; 34: e108. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

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