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Mutagenesis


This category presents widely useful methods for random or site-specific, chemical or enzymatic, in vitro or in vivo mutagenesis.


Citations 21-30 of 81 total displayed.

Past content

Methods Online
Genetic manipulation of an exogenous non-immunoglobulin protein by gene conversion machinery in a chicken B cell line
Naoki Kanayama, Kagefumi Todo, Satoko Takahashi, Masaki Magari, and Hitoshi Ohmori
Nucleic Acids Res. 2006; 34: e10. [Abstract] [Full text] [Print PDF]  

Methods Online
Improved methods for the generation of human gene knockout and knockin cell lines
Ozlem Topaloglu, Paula J. Hurley, Ozlem Yildirim, Curt I. Civin, and Fred Bunz
Nucleic Acids Res. 2005; 33: e158. [Abstract] [Full text] [Print PDF]  

Methods Online
Characterization of zebrafish Rad52 and replication protein A for oligonucleotide-mediated mutagenesis
Nobuhiro Takahashi and Igor B. Dawid
Nucleic Acids Res. 2005; 33: e120. [Abstract] [Full text] [Print PDF]  

Methods Online
Nucleotide exchange and excision technology (NExT) DNA shuffling: a robust method for DNA fragmentation and directed evolution
Kristian M. Müller, Sabine C. Stebel, Susanne Knall, Gregor Zipf, Hubert S. Bernauer, and Katja M. Arndt
Nucleic Acids Res. 2005; 33: e117. [Abstract] [Full text] [Print PDF]  

Methods Online
A novel PCR strategy for high-efficiency, automated site-directed mutagenesis
Wu Wu, Zongchao Jia, Ping Liu, Zhigang Xie, and Qun Wei
Nucleic Acids Res. 2005; 33: e110. [Abstract] [Full text] [Print PDF]  

Methods Online
A novel method of screening thrombin-inhibiting DNA aptamers using an evolution-mimicking algorithm
Kazunori Ikebukuro, Yuji Okumura, Koichi Sumikura, and Isao Karube
Nucleic Acids Res. 2005; 33: e108. [Abstract] [Full text] [Print PDF]  

Methods Online
Offset recombinant PCR: a simple but effective method for shuffling compact heterologous domains
David A. Rozak and Philip N. Bryan
Nucleic Acids Res. 2005; 33: e82. [Abstract] [Full text] [Print PDF]  

Methods Online
Triplet nucleotide removal at random positions in a target gene: the tolerance of TEM-1 ß-lactamase to an amino acid deletion
D. Dafydd Jones
Nucleic Acids Res. 2005; 33: e80. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Correcting errors in synthetic DNA through consensus shuffling
Brock F. Binkowski, Kathryn E. Richmond, James Kaysen, Michael R. Sussman, and Peter J. Belshaw
Nucleic Acids Res. 2005; 33: e55. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Suppression of nonsense-mediated mRNA decay permits unbiased gene trapping in mouse embryonic stem cells
Toshiaki Shigeoka, Masashi Kawaichi, and Yasumasa Ishida
Nucleic Acids Res. 2005; 33: e20. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

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