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Mutagenesis


This category presents widely useful methods for random or site-specific, chemical or enzymatic, in vitro or in vivo mutagenesis.


Citations 41-50 of 81 total displayed.

Past content

NAR Methods Online
Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution
Tuck Seng Wong, Kang Lan Tee, Berhard Hauer, and Ulrich Schwaneberg
Nucleic Acids Res. 2004; 32: e26. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
High efficiency, site-specific excision of a marker gene by the phage P1 cre–loxP system in the yellow fever mosquito, Aedes aegypti
Nijole Jasinskiene, Craig J. Coates, Aurora Ashikyan, and Anthony A. James
Nucleic Acids Res. 2003; 31: e147. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Method to protect a targeted amino acid residue during random mutagenesis
Daisuke Umeno, Kaori Hiraga, and Frances H. Arnold
Nucleic Acids Res. 2003; 31: e91. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A simple two-step, ‘hit and fix’ method to generate subtle mutations in BACs using short denatured PCR fragments
Yongping Yang and Shyam K. Sharan
Nucleic Acids Res. 2003; 31: e80. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
An inbred 129SvEv GFPCre transgenic mouse that deletes loxP-flanked genes in all tissues
John R. Scheel, Lisa J. Garrett, Duane M. Allen, Todd A. Carter, Lynne Randolph-Moore, Micheal J. Gambello, Fred H. Gage, Anthony Wynshaw-Boris, and Carrolee Barlow
Nucleic Acids Res. 2003; 31: e57. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Targeted gene modification in mismatch-repair-deficient embryonic stem cells by single-stranded DNA oligonucleotides
Marleen Dekker, Conny Brouwers, and Hein te Riele
Nucleic Acids Res. 2003; 31: e27. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Cre-mediated germline mosaicism: a new transgenic mouse for the selective removal of residual markers from tri-lox conditional alleles
Patricia Leneuve, Sabine Colnot, Ghislaine Hamard, Fiona Francis, Michiko Niwa-Kawakita, Marco Giovannini, and Martin Holzenberger
Nucleic Acids Res. 2003; 31: e21. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
De novo production of diverse intracellular antibody libraries
Tomoyuki Tanaka, Grace T. Y. Chung, Alan Forster, M. Natividad Lobato, and Terence H. Rabbitts
Nucleic Acids Res. 2003; 31: e23. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Rapid generation of inducible mouse mutants
Jost Seibler, Branko Zevnik, Birgit Küter-Luks, Susanne Andreas, Heidrun Kern, Thomas Hennek, Anja Rode, Cornelia Heimann, Nicole Faust, Gunther Kauselmann, Michael Schoor, Rudolf Jaenisch, Klaus Rajewsky, Ralf Kühn, and Frieder Schwenk
Nucleic Acids Res. 2003; 31: e12. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening
Lei Young and Qihan Dong
Nucleic Acids Res. 2003; 31: e11. [Abstract] [Full text] [Print PDF]  

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