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Mutagenesis


This category presents widely useful methods for random or site-specific, chemical or enzymatic, in vitro or in vivo mutagenesis.


Citations 71-80 of 81 total displayed.

Past content

Articles
A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis
Matthew C. Biery, Fiona J. Stewart, Anne E. Stellwagen, Elisabeth A. Raleigh, and Nancy L. Craig
Nucleic Acids Res. 2000; 28: 1067-1077. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Targeted mutagenesis of DNA with alkylating RecA assisted oligonucleotides
MJ Singer, MA Podyminogin, MA Metcalf, MW Reed, DA Brown, HB Gamper, RB Meyer, and RM Wydro
Nucleic Acids Res. 1999; 27: e38. [Abstract] [Full text] [Print PDF]  

Articles
Gene targeted DNA double-strand break induction by (125)I-labeled triplex-forming oligonucleotides is highly mutagenic following repair in human cells
K Mezhevaya, TA Winters, and RD Neumann
Nucleic Acids Res. 1999; 27: 4282-4290. [Abstract] [Full text] [Print PDF]  

Articles
In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis
TJ Griffin, 4th, L Parsons, AE Leschziner, J DeVost, KM Derbyshire, and ND Grindley
Nucleic Acids Res. 1999; 27: 3859-3865. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Simplified generation of targeting constructs using ET recombination
PO Angrand, N Daigle, F van der Hoeven, HR Scholer, and AF Stewart
Nucleic Acids Res. 1999; 27: e16. [Abstract] [Full text] [Print PDF]  

Articles
An efficient and accurate integration of mini-Mu transposons in vitro: a general methodology for functional genetic analysis and molecular biology applications
S Haapa, S Taira, E Heikkinen, and H Savilahti
Nucleic Acids Res. 1999; 27: 2777-2784. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Targeted deletions created in yeast vectors by recombinational excision
M Duno, C Bendixen, L Krejci, and B Thomsen
Nucleic Acids Res. 1999; 27: e1. [Abstract] [Full text]  

Articles
Transplacement mutagenesis: a novel in situ mutagenesis system using phage-plasmid recombination
MW Unger, SY Liu, and DE Rancourt
Nucleic Acids Res. 1999; 27: 1480-1484. [Abstract] [Full text]  

Articles
Gene replacement with linear DNA in electroporated wild-type Escherichia coli
M El Karoui, SK Amundsen, P Dabert, and A Gruss
Nucleic Acids Res. 1999; 27: 1296-1299. [Abstract] [Full text] [Print PDF]  

Articles
Random mutagenesis by recombinational capture of PCR products in Bacillus subtilis and Acinetobacter calcoaceticus
A Melnikov and PJ Youngman
Nucleic Acids Res. 1999; 27: 1056-1062. [Abstract] [Full text] [Print PDF]  

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