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New restriction enzymes


This category includes descriptions of new restriction enzymes that either recognize novel sequences or cleave at new positions within known recognition sites (neoschizomers).


Citations 1-10 of 27 total displayed.

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Methods Online
Using shotgun sequence data to find active restriction enzyme genes
Yu Zheng, Janos Posfai, Richard D. Morgan, Tamas Vincze, and Richard J. Roberts
Nucleic Acids Res. 2009; 37: e1. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Past content

Methods Online
Quick identification of Type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids
Junichi Ryu and Edward Rowsell
Nucleic Acids Res. 2008; 36: e81. [Abstract] [Full text] [Print PDF]  

Methods Online
The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases
Hua Wei, Caitlin Therrien, Aine Blanchard, Shengxi Guan, and Zhenyu Zhu
Nucleic Acids Res. 2008; 36: e50. [Abstract] [Full text] [Print PDF]  

Methods Online
Selection of restriction endonucleases using artificial cells
Yu Zheng and Richard J. Roberts
Nucleic Acids Res. 2007; 35: e83. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences
Julianne Smith, Sylvestre Grizot, Sylvain Arnould, Aymeric Duclert, Jean-Charles Epinat, Patrick Chames, Jesús Prieto, Pilar Redondo, Francisco J. Blanco, Jerónimo Bravo, Guillermo Montoya, Frédéric Pâques, and Philippe Duchateau
Nucleic Acids Res. 2006; 34: e149. [Abstract] [Full text] [Print PDF]  

Methods Online
In vivo selection of engineered homing endonucleases using double-strand break induced homologous recombination
Patrick Chames, Jean-Charles Epinat, Sophie Guillier, Amélie Patin, Emmanuel Lacroix, and Frédéric Pâques
Nucleic Acids Res. 2005; 33: e178. [Abstract] [Full text] [Print PDF]  

Methods Online
I-ApeI: a novel intron-encoded LAGLIDADG homing endonuclease from the archaeon, Aeropyrum pernix K1
Norimichi Nomura, Yayoi Morinaga, Nobuaki Shirai, and Yoshihiko Sako
Nucleic Acids Res. 2005; 33: e116. [Abstract] [Full text] [Print PDF]  

Methods Online
Four new type I restriction enzymes identified in Escherichia coli clinical isolates
Julie K. A. Kasarjian, Yoshiaki Kodama, Masatake Iida, Katsura Matsuda, and Junichi Ryu
Nucleic Acids Res. 2005; 33: e114. [Abstract] [Full text] [Print PDF]  

Methods Online
Discovery of a novel restriction endonuclease by genome comparison and application of a wheat-germ-based cell-free translation assay: PabI (5'-GTA/C) from the hyperthermophilic archaeon Pyrococcus abyssi
Ken Ishikawa, Miki Watanabe, Toshihiro Kuroita, Ikuo Uchiyama, Janusz M. Bujnicki, Bunsei Kawakami, Masaru Tanokura, and Ichizo Kobayashi
Nucleic Acids Res. 2005; 33: e112. [Abstract] [Full text] [Print PDF]  

Methods Online
KpnBI is the prototype of a new family (IE) of bacterial type I restriction-modification system
V. Chin, V. Valinluck, S. Magaki, and J. Ryu
Nucleic Acids Res. 2004; 32: e138. [Abstract] [Full text] [Print PDF]  

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