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Nucleic acid amplification
This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.
Citations 1-10 of 117 total displayed.
- Methods Online
The use of multiple displacement amplification to amplify complex DNA libraries
- Melissa J. Fullwood, Jack J. S. Tan, Patrick W. P. Ng, Kuo Ping Chiu, Jun Liu, Chia Lin Wei, and Yijun Ruan
Nucleic Acids Res. 2008; 36: e32.
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- Methods Online
Codon choice in genes depends on flanking sequence information—implications for theoretical reverse translation
- Karthikeyan Sivaraman, AswinSaiNarain Seshasayee, Patrick M. Tarwater, and Alexander M. Cole
Nucleic Acids Res. 2008; 36: e16.
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- Methods Online
From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing
- Matthias Meyer, Adrian W. Briggs, Tomislav Maricic, Barbara Höber, Barbara Höffner, Johannes Krause, Antje Weihmann, Svante Pääbo, and Michael Hofreiter
Nucleic Acids Res. 2008; 36: e5.
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- Methods Online
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature
- Haukur Gudnason, Martin Dufva, D.D. Bang, and Anders Wolff
Nucleic Acids Res. 2007; 35: e127.
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- Methods Online
FLAG assay as a novel method for real-time signal generation during PCR: application to detection and genotyping of KRAS codon 12 mutations
- Giulia Amicarelli, Erlet Shehi, G. Mike Makrigiorgos, and Daniel Adlerstein
Nucleic Acids Res. 2007; 35: e131.
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- Methods Online
Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)
- Traci Kiesling, Kendra Cox, Eugene A. Davidson, Kenneth Dretchen, Guy Grater, Shannon Hibbard, Roger S. Lasken, Jonathan Leshin, Evan Skowronski, and Mark Danielsen
Nucleic Acids Res. 2007; 35: e117.
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- Methods Online
Short pyrosequencing reads suffice for accurate microbial community analysis
- Zongzhi Liu, Catherine Lozupone, Micah Hamady, Frederic D. Bushman, and Rob Knight
Nucleic Acids Res. 2007; 35: e120.
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- Methods Online
Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR
- Shihai Huang, John Salituro, Ning Tang, Ka-Cheung Luk, John Hackett, Jr, Priscilla Swanson, Gavin Cloherty, Wai-Bing Mak, John Robinson, and Klara Abravaya
Nucleic Acids Res. 2007; 35: e101.
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- Methods Online
Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)
- Olivier Loudig, Ekaterina Milova, Margaret Brandwein-Gensler, Aldo Massimi, Thomas J. Belbin, Geoffrey Childs, Robert H. Singer, Thomas Rohan, and Michael B. Prystowsky
Nucleic Acids Res. 2007; 35: e94.
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- Methods Online
Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry
- Eva Kühn-Hölsken, Olexandr Dybkov, Björn Sander, Reinhard Lührmann, and Henning Urlaub
Nucleic Acids Res. 2007; 35: e95.
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