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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 1-10 of 140 total displayed.

Most recent content

Methods Online
Analysis of splicing patterns by pyrosequencing
Agnès Méreau, Vincent Anquetil, Marie Cibois, Maud Noiret, Aline Primot, Audrey Vallée, and Luc Paillard
Nucleic Acids Res. 2009; 37: e126. [Abstract] [Full text] [Print PDF]  

Methods Online
Zip Nucleic Acids: new high affinity oligonucleotides as potent primers for PCR and reverse transcription
Valérie Moreau, Emilie Voirin, Clément Paris, Mitsuharu Kotera, Marc Nothisen, Jean-Serge Rémy, Jean-Paul Behr, Patrick Erbacher, and Nathalie Lenne-Samuel
Nucleic Acids Res. 2009; 37: e130. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Past content

Methods Online
Novel method for high-throughput colony PCR screening in nanoliter-reactors
Marcel Walser, Rene Pellaux, Andreas Meyer, Matthias Bechtold, Herve Vanderschuren, Richard Reinhardt, Joseph Magyar, Sven Panke, and Martin Held
Nucleic Acids Res. 2009; 37: e57. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Computational Biology
PrimerHunter: a primer design tool for PCR-based virus subtype identification
Jorge Duitama, Dipu Mohan Kumar, Edward Hemphill, Mazhar Khan, Ion I. Mandoiu, and Craig E. Nelson
Nucleic Acids Res. 2009; 37: 2483-2492. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Mutants of Taq DNA polymerase resistant to PCR inhibitors allow DNA amplification from whole blood and crude soil samples
Milko B. Kermekchiev, Lyubka I. Kirilova, Erika E. Vail, and Wayne M. Barnes
Nucleic Acids Res. 2009; 37: e40. [Abstract] [Full text] [Print PDF]  

Methods Online
Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data
J. M. Ruijter, C. Ramakers, W. M. H. Hoogaars, Y. Karlen, O. Bakker, M. J. B. van den Hoff, and A. F. M. Moorman
Nucleic Acids Res. 2009; 37: e45. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis
Hongye Ye, Mo Chao Huang, Mo-Huang Li, and Jackie Y. Ying
Nucleic Acids Res. 2009; 37: e51. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Sensitive isothermal detection of nucleic-acid sequence by primer generation–rolling circle amplification
Taku Murakami, Jun Sumaoka, and Makoto Komiyama
Nucleic Acids Res. 2009; 37: e19. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement polymerization reaction
Qiuping Guo, Xiaohai Yang, Kemin Wang, Weihong Tan, Wei Li, Hongxing Tang, and Huimin Li
Nucleic Acids Res. 2009; 37: e20. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Telomere length measurement by a novel monochrome multiplex quantitative PCR method
Richard M. Cawthon
Nucleic Acids Res. 2009; 37: e21. [Abstract] [Full text] [Print PDF]  

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