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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 91-100 of 140 total displayed.

Past content

NAR Methods Online
Amplification of repeat-containing transcribed sequences (ARTS): a transcriptome fingerprinting strategy to detect functionally relevant microsatellite mutations in cancer
Martina Olivero, Tina Ruggiero, Nadia Coltella, Antonella Maffe’, Raffaele Calogero, Enzo Medico, and Maria Flavia Di Renzo
Nucleic Acids Res. 2003; 31: e33. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Mechanisms underlying the impact of humic acids on DNA quantification by SYBR Green I and consequences for the analysis of soils and aquatic sediments
Hubert Zipper, Christiane Buta, Katrin Lämmle, Herwig Brunner, Jürgen Bernhagen, and Frank Vitzthum
Nucleic Acids Res. 2003; 31: e39. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Novel amplification of DNA in a hairpin structure: towards a radical elimination of PCR errors from amplified DNA
Manjit Kaur and G. Mike Makrigiorgos
Nucleic Acids Res. 2003; 31: e26. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Real-time monitoring of rolling-circle amplification using a modified molecular beacon design
Mats Nilsson, Mats Gullberg, Fredrik Dahl, Karoly Szuhai, and Anton K. Raap
Nucleic Acids Res. 2002; 30: e66. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification
Jan P. Schouten, Cathal J. McElgunn, Raymond Waaijer, Danny Zwijnenburg, Filip Diepvens, and Gerard Pals
Nucleic Acids Res. 2002; 30: e57. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Integration of DNA ligation and rolling circle amplification for the homogeneous, end-point detection of single nucleotide polymorphisms
Judith Pickering, Anona Bamford, Varsha Godbole, Jackie Briggs, Giuseppe Scozzafava, Phyllida Roe, Claire Wheeler, Firman Ghouze, and Sarah Cuss
Nucleic Acids Res. 2002; 30: e60. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Direct micro-haplotyping by multiple double PCR amplifications of specific alleles (MD-PASA)
Yuval Eitan and Yechezkel Kashi
Nucleic Acids Res. 2002; 30: e62. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis
David M. Hoover and Jacek Lubkowski
Nucleic Acids Res. 2002; 30: e43. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore
Irina Nazarenko, Brian Lowe, Marlene Darfler, Pranvera Ikonomi, David Schuster, and Ayoub Rashtchian
Nucleic Acids Res. 2002; 30: e37. [Abstract] [Full text] [Print PDF]  

Articles
Heteroduplexes in mixed-template amplifications: formation, consequence and elimination by ‘reconditioning PCR’
Janelle R. Thompson, Luisa A. Marcelino, and Martin F. Polz
Nucleic Acids Res. 2002; 30: 2083-2088. [Abstract] [Full text] [Print PDF]  

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