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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 101-110 of 140 total displayed.

Past content

NAR Methods Online
Principles of quantitation of viral loads using nucleic acid sequence-based amplification in combination with homogeneous detection using molecular beacons
Jos J. A. M. Weusten, Wim M. Carpay, Tom A. M. Oosterlaken, Martien C. A. van Zuijlen, and Paul A. van de Wiel
Nucleic Acids Res. 2002; 30: e26. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Enzymatic repair of selected cross-linked homoduplex molecules enhances nuclear gene rescue from Pompeii and Herculaneum remains
Giovanni Di Bernardo, Stefania Del Gaudio, Marcella Cammarota, Umberto Galderisi, Antonino Cascino, and Marilena Cipollaro
Nucleic Acids Res. 2002; 30: e16. [Abstract] [Full text] [Print PDF]  

Articles
RT–PCR analysis of 5' to 3'-end-ligated mRNAs identifies the extremities of cox2 transcripts in pea mitochondria
Josef Kuhn and Stefan Binder
Nucleic Acids Res. 2002; 30: 439-446. [Abstract] [Full text] [Print PDF]  

Articles
Quantitative amplification of single-stranded DNA (QAOS) demonstrates that cdc13-1 mutants generate ssDNA in a telomere to centromere direction
Claire Booth, Elen Griffith, Gerard Brady, and David Lydall
Nucleic Acids Res. 2001; 29: 4414-4422. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Duplex Scorpion primers in SNP analysis and FRET applications
Antonio Solinas, Lynda J. Brown, Catherine McKeen, John M. Mellor, Jamie Nicol, Nicky Thelwell, and Tom Brown
Nucleic Acids Res. 2001; 29: e96. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
C-SPACE (cleavage-specific amplification of cDNA ends): a novel method of ribozyme-mediated gene identification
Martin Krüger, Carmela Beger, Peter J. Welch, Jack R. Barber, and Flossie Wong-Staal
Nucleic Acids Res. 2001; 29: e94. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Quantification of splice variants using real-time PCR
Ina I. Vandenbroucke, Jo Vandesompele, Anne De Paepe, and Ludwine Messiaen
Nucleic Acids Res. 2001; 29: e68. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A new G-tailing method for the determination of the poly(A) tail length applied to hepatitis A virus RNA
Yuri Yu. Kusov, Gocha Shatirishvili, Georgy Dzagurov, and Verena Gauss-Müller
Nucleic Acids Res. 2001; 29: e57. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Specific detection of DNA and RNA targets using a novel isothermal nucleic acid amplification assay based on the formation of a three-way junction structure
Susan D. Wharam, Peter Marsh, John S. Lloyd, Trevor D. Ray, Graham A. Mock, René Assenberg, Julie E. McPhee, Philip Brown, Anthony Weston, and Donald L. N. Cardy
Nucleic Acids Res. 2001; 29: e54. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A new mathematical model for relative quantification in real-time RT–PCR
Michael W. Pfaffl
Nucleic Acids Res. 2001; 29: e45. [Abstract] [Full text] [Print PDF]  

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