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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 11-20 of 140 total displayed.

Past content

Methods Online
A single molecule array for digital targeted molecular analyses
Jenny Göransson, Carolina Wählby, Magnus Isaksson, W. Mathias Howell, Jonas Jarvius, and Mats Nilsson
Nucleic Acids Res. 2009; 37: e7. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance
Alexandre V. Lebedev, Natasha Paul, Joyclyn Yee, Victor A. Timoshchuk, Jonathan Shum, Kei Miyagi, Jack Kellum, Richard I. Hogrefe, and Gerald Zon
Nucleic Acids Res. 2008; 36: e131. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Analysis of copy number variation using quantitative interspecies competitive PCR
Nigel M. Williams, Hywel Williams, Elisa Majounie, Nadine Norton, Beate Glaser, Huw R. Morris, Michael J. Owen, and Michael C. O’Donovan
Nucleic Acids Res. 2008; 36: e112. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Advantages of q-PCR as a method of screening for gene targeting in mammalian cells using conventional and whole BAC-based constructs
Julio Gómez-Rodríguez, Valance Washington, Jun Cheng, Amalia Dutra, Evgenia Pak, Pentao Liu, Daniel W. McVicar, and Pamela L. Schwartzberg
Nucleic Acids Res. 2008; 36: e117. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
NAIMA: target amplification strategy allowing quantitative on-chip detection of GMOs
Dany Morisset, David Dobnik, Sandrine Hamels, Jana Zel, and Kristina Gruden
Nucleic Acids Res. 2008; 36: e118. [Abstract] [Full text] [Print PDF]  

Methods Online
Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Gouse M. Shaik, Lubica Dráberová, Peter Dráber, Michael Boubelík, and Petr Dráber
Nucleic Acids Res. 2008; 36: e93. [Abstract] [Full text] [Print PDF]  

Methods Online
Specific ligation to double-stranded RNA for analysis of cellular RNA::RNA interactions
Omid R. Faridani, Gerald M. McInerney, Katarina Gradin, and Liam Good
Nucleic Acids Res. 2008; 36: e99. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Primase-based whole genome amplification
Ying Li, Hyun-Jin Kim, Chunyang Zheng, Wing Huen A. Chow, Jeonghwa Lim, Brendan Keenan, Xiaojing Pan, Bertrand Lemieux, and Huimin Kong
Nucleic Acids Res. 2008; 36: e79. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A new method for robust quantitative and qualitative analysis of real-time PCR
Eric B. Shain and John M. Clemens
Nucleic Acids Res. 2008; 36: e91. [Abstract] [Full text] [Print PDF]  

Methods Online
Inversing the natural hydrogen bonding rule to selectively amplify GC-rich ADAR-edited RNAs
Rodolphe Suspène, Myrtille Renard, Michel Henry, Denise Guétard, David Puyraimond-Zemmour, Agnès Billecocq, Michèle Bouloy, Frederic Tangy, Jean-Pierre Vartanian, and Simon Wain-Hobson
Nucleic Acids Res. 2008; 36: e72. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

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