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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 21-30 of 140 total displayed.

Past content

Methods Online
Development of a single tube 640-plex genotyping method for detection of nucleic acid variations on microarrays
Kaarel Krjutskov, Reidar Andreson, Reedik Mägi, Tiit Nikopensius, Andrey Khrunin, Evelin Mihailov, Veronika Tammekivi, Helena Sork, Maido Remm, and Andres Metspalu
Nucleic Acids Res. 2008; 36: e75. [Abstract] [Full text] [Print PDF]  

Methods Online
UniPrime: a workflow-based platform for improved universal primer design
Michaël Bekaert and Emma C. Teeling
Nucleic Acids Res. 2008; 36: e56. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Computational Biology
MultiPriDe: automated batch development of quantitative real-time PCR primers
A. C. Ziesel, M. A. Chrenek, and P. W. Wong
Nucleic Acids Res. 2008; 36: 3095-3100. [Abstract] [Full text] [Print PDF]  

Methods Online
The use of multiple displacement amplification to amplify complex DNA libraries
Melissa J. Fullwood, Jack J. S. Tan, Patrick W. P. Ng, Kuo Ping Chiu, Jun Liu, Chia Lin Wei, and Yijun Ruan
Nucleic Acids Res. 2008; 36: e32. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Codon choice in genes depends on flanking sequence information—implications for theoretical reverse translation
Karthikeyan Sivaraman, AswinSaiNarain Seshasayee, Patrick M. Tarwater, and Alexander M. Cole
Nucleic Acids Res. 2008; 36: e16. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing
Matthias Meyer, Adrian W. Briggs, Tomislav Maricic, Barbara Höber, Barbara Höffner, Johannes Krause, Antje Weihmann, Svante Pääbo, and Michael Hofreiter
Nucleic Acids Res. 2008; 36: e5. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature
Haukur Gudnason, Martin Dufva, D.D. Bang, and Anders Wolff
Nucleic Acids Res. 2007; 35: e127. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
FLAG assay as a novel method for real-time signal generation during PCR: application to detection and genotyping of KRAS codon 12 mutations
Giulia Amicarelli, Erlet Shehi, G. Mike Makrigiorgos, and Daniel Adlerstein
Nucleic Acids Res. 2007; 35: e131. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA)
Traci Kiesling, Kendra Cox, Eugene A. Davidson, Kenneth Dretchen, Guy Grater, Shannon Hibbard, Roger S. Lasken, Jonathan Leshin, Evan Skowronski, and Mark Danielsen
Nucleic Acids Res. 2007; 35: e117. [Abstract] [Full text] [Print PDF]  

Methods Online
Short pyrosequencing reads suffice for accurate microbial community analysis
Zongzhi Liu, Catherine Lozupone, Micah Hamady, Frederic D. Bushman, and Rob Knight
Nucleic Acids Res. 2007; 35: e120. [Abstract] [Full text] [Print PDF]  

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