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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 31-40 of 140 total displayed.

Past content

Methods Online
Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR
Shihai Huang, John Salituro, Ning Tang, Ka-Cheung Luk, John Hackett, Jr, Priscilla Swanson, Gavin Cloherty, Wai-Bing Mak, John Robinson, and Klara Abravaya
Nucleic Acids Res. 2007; 35: e101. [Abstract] [Full text] [Print PDF]  

Methods Online
Molecular restoration of archived transcriptional profiles by complementary-template reverse-transcription (CT-RT)
Olivier Loudig, Ekaterina Milova, Margaret Brandwein-Gensler, Aldo Massimi, Thomas J. Belbin, Geoffrey Childs, Robert H. Singer, Thomas Rohan, and Michael B. Prystowsky
Nucleic Acids Res. 2007; 35: e94. [Abstract] [Full text] [Print PDF]  

Methods Online
Improved identification of enriched peptide–RNA cross-links from ribonucleoprotein particles (RNPs) by mass spectrometry
Eva Kühn-Hölsken, Olexandr Dybkov, Björn Sander, Reinhard Lührmann, and Henning Urlaub
Nucleic Acids Res. 2007; 35: e95. [Abstract] [Full text] [Print PDF]  

Methods Online
Quantitative multiplexing analysis of PCR-amplified ribosomal RNA genes by hierarchical oligonucleotide primer extension reaction
Jer-Horng Wu and Wen-Tso Liu
Nucleic Acids Res. 2007; 35: e82. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Molecular Biology
Recovery of bisulfite-converted genomic sequences in the methylation-sensitive QPCR
Kristofer Munson, Jarrod Clark, Katarzyna Lamparska-Kupsik, and Steven S. Smith
Nucleic Acids Res. 2007; 35: 2893-2903. [Abstract] [Full text] [Print PDF]  

Methods Online
Multiplex amplification of all coding sequences within 10 cancer genes by Gene-Collector
Simon Fredriksson, Johan Banér, Fredrik Dahl, Angela Chu, Hanlee Ji, Katrina Welch, and Ronald W. Davis
Nucleic Acids Res. 2007; 35: e47. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Dual priming oligonucleotide system for the multiplex detection of respiratory viruses and SNP genotyping of CYP2C19 gene
Jong-Yoon Chun, Kyoung-Joong Kim, In-Taek Hwang, Yun-Jee Kim, Dae-Hoon Lee, In-Kyoung Lee, and Jong-Kee Kim
Nucleic Acids Res. 2007; 35: e40. [Abstract] [Full text] [Print PDF]  

Methods Online
Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer
Kenji Kinoshita, Kentaro Fujimoto, Toru Yakabe, Shin Saito, Yuzo Hamaguchi, Takayuki Kikuchi, Ken Nonaka, Shigenori Murata, Daisuke Masuda, Wataru Takada, Sohei Funaoka, Susumu Arai, Hisao Nakanishi, Kanehisa Yokoyama, Kazuhiko Fujiwara, and Kenichi Matsubara
Nucleic Acids Res. 2007; 35: e3. [Abstract] [Full text] [Print PDF]  

Computational Biology
Greene SCPrimer: a rapid comprehensive tool for designing degenerate primers from multiple sequence alignments
Omar J. Jabado, Gustavo Palacios, Vishal Kapoor, Jeffrey Hui, Neil Renwick, Junhui Zhai, Thomas Briese, and W. Ian Lipkin
Nucleic Acids Res. 2006; 34: 6605-6611. [Abstract] [Full text] [Print PDF]  

Methods Online
Position-dependent effects of locked nucleic acid (LNA) on DNA sequencing and PCR primers
Joshua D. Levin, Dean Fiala, Meinrado F. Samala, Jason D. Kahn, and Raymond J. Peterson
Nucleic Acids Res. 2006; 34: e142. [Abstract] [Full text] [Print PDF]  

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