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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 41-50 of 140 total displayed.

Past content

Methods Online
A DNA biochip for on-the-spot multiplexed pathogen identification
Siu-Wai Yeung, Thomas Ming-Hung Lee, Hong Cai, and I-Ming Hsing
Nucleic Acids Res. 2006; 34: e118. [Abstract] [Full text] [Print PDF]  

Methods Online
Nanoliter high throughput quantitative PCR
Tom Morrison, James Hurley, Javier Garcia, Karl Yoder, Arrin Katz, Douglas Roberts, Jamie Cho, Tanya Kanigan, Sergey E. Ilyin, Daniel Horowitz, James M. Dixon, and Colin J.H. Brenan
Nucleic Acids Res. 2006; 34: e123. [Abstract] [Full text] [Print PDF]  

Methods Online
Tapping diversity lost in transformations—in vitro amplification of ligation reactions
Daniel Christ, Kristoffer Famm, and Greg Winter
Nucleic Acids Res. 2006; 34: e108. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Simultaneous amplification and screening of whole plasmids using the T7 bacteriophage replisome
Yan Xu, Hyun-jin Kim, Ann Kays, Jared Rice, and Huimin Kong
Nucleic Acids Res. 2006; 34: e98. [Abstract] [Full text] [Print PDF]  

Methods Online
Molecular Zipper: a fluorescent probe for real-time isothermal DNA amplification
Jizu Yi, Wandi Zhang, and David Y. Zhang
Nucleic Acids Res. 2006; 34: e81. [Abstract] [Full text] [Print PDF]  

Methods Online
Short, synthetic and selectively 13C-labeled RNA sequences for the NMR structure determination of protein–RNA complexes
Philipp Wenter, Luc Reymond, Sigrid D. Auweter, Frédéric H.-T. Allain, and Stefan Pitsch
Nucleic Acids Res. 2006; 34: e79. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes
Pavel Neuzil, Chunyan Zhang, Juergen Pipper, Sharon Oh, and Lang Zhuo
Nucleic Acids Res. 2006; 34: e77. [Abstract] [Full text] [Print PDF]  

Methods Online
Primer Extension Enrichment Reaction (PEER): a new subtraction method for identification of genetic differences between biological specimens
Lilia Ganova-Raeva, Xinjian Zhang, Fengli Cao, Howard Fields, and Yury Khudyakov
Nucleic Acids Res. 2006; 34: e76. [Abstract] [Full text] [Print PDF]  

Methods Online
Improvements of rolling circle amplification (RCA) efficiency and accuracy using Thermus thermophilus SSB mutant protein
Jin Inoue, Yasushi Shigemori, and Tsutomu Mikawa
Nucleic Acids Res. 2006; 34: e69. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A real-time PCR-based method for determining the surface coverage of thiol-capped oligonucleotides bound onto gold nanoparticles
Eun-Young Kim, Jennifer Stanton, Rafael A. Vega, Kevin J. Kunstman, Chad A. Mirkin, and Steven M. Wolinsky
Nucleic Acids Res. 2006; 34: e54. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

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