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Nucleic acid amplification
This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.
Citations 51-60 of 140 total displayed.
- Methods Online
BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies
- Milan Fedurco, Anthony Romieu, Scott Williams, Isabelle Lawrence, and Gerardo Turcatti
Nucleic Acids Res. 2006; 34: e22.
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- Methods Online
MicroRNA expression profiling of single whole embryonic stem cells
- Fuchou Tang, Petra Hajkova, Sheila C. Barton, Kaiqin Lao, and M. Azim Surani
Nucleic Acids Res. 2006; 34: e9.
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- Methods Online
Enhancing the efficiency of a PCR using gold nanoparticles
- Min Li, Yu-Cheng Lin, Chao-Chin Wu, and Hsiao-Sheng Liu
Nucleic Acids Res. 2005; 33: e184.
[Abstract]
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- Methods Online
Faster quantitative real-time PCR protocols may lose sensitivity and show increased variability
- Chelsey Hilscher, Wolfgang Vahrson, and Dirk P. Dittmer
Nucleic Acids Res. 2005; 33: e182.
[Abstract]
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- Methods Online
Real-time quantification of microRNAs by stem–loop RT–PCR
- Caifu Chen, Dana A. Ridzon, Adam J. Broomer, Zhaohui Zhou, Danny H. Lee, Julie T. Nguyen, Maura Barbisin, Nan Lan Xu, Vikram R. Mahuvakar, Mark R. Andersen, Kai Qin Lao, Kenneth J. Livak, and Karl J. Guegler
Nucleic Acids Res. 2005; 33: e179.
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- Methods Online
PCR inhibition by reverse transcriptase leads to an overestimation of amplification efficiency
- Oleg Suslov and Dennis A. Steindler
Nucleic Acids Res. 2005; 33: e181.
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- Methods Online
Miniature RT–PCR system for diagnosis of RNA-based viruses
- Chia-Sheng Liao, Gwo-Bin Lee, Hsiao-Sheng Liu, Tsung-Min Hsieh, and Ching-Hsing Luo
Nucleic Acids Res. 2005; 33: e156.
[Abstract]
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- Methods Online
PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
- Takaaki Kojima, Yoshiaki Takei, Miharu Ohtsuka, Yasuaki Kawarasaki, Tsuneo Yamane, and Hideo Nakano
Nucleic Acids Res. 2005; 33: e150.
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- Methods Online
End invasion of peptide nucleic acids (PNAs) with mixed-base composition into linear DNA duplexes
- Irina V. Smolina, Vadim V. Demidov, Viatcheslav A. Soldatenkov, Sergey G. Chasovskikh, and Maxim D. Frank-Kamenetskii
Nucleic Acids Res. 2005; 33: e146.
[Abstract]
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- Methods Online
Headloop suppression PCR and its application to selective amplification of methylated DNA sequences
- Keith N. Rand, Thu Ho, Wenjia Qu, Susan M. Mitchell, Rose White, Susan J. Clark, and Peter L. Molloy
Nucleic Acids Res. 2005; 33: e127.
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