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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 61-70 of 140 total displayed.

Past content

Methods Online
Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products
Yasushi Shigemori, Tsutomu Mikawa, Takehiko Shibata, and Michio Oishi
Nucleic Acids Res. 2005; 33: e126. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Genome-wide selection of unique and valid oligonucleotides
Heikki Hyyrö, Martti Juhola, and Mauno Vihinen
Nucleic Acids Res. 2005; 33: e115. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Genome amplification of single sperm using multiple displacement amplification
Zhengwen Jiang, Xingqi Zhang, Ranjan Deka, and Li Jin
Nucleic Acids Res. 2005; 33: e91. [Abstract] [Full text] [Print PDF]  

Methods Online
PieceMaker: selection of DNA fragments for selector-guided multiplex amplification
Johan Stenberg, Fredrik Dahl, Ulf Landegren, and Mats Nilsson
Nucleic Acids Res. 2005; 33: e72. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
Fredrik Dahl, Mats Gullberg, Johan Stenberg, Ulf Landegren, and Mats Nilsson
Nucleic Acids Res. 2005; 33: e71. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Methylation profiles of genes utilizing newly developed CpG island methylation microarray on colorectal cancer patients
Naoki Kimura, Takeshi Nagasaka, Jun Murakami, Hiromi Sasamoto, Masahiro Murakami, Noriaki Tanaka, and Nagahide Matsubara
Nucleic Acids Res. 2005; 33: e46. [Abstract] [Full text] [Print PDF]  

Methods Online
Selection of genomic sequences that bind tightly to Ff gene 5 protein: primer-free genomic SELEX
Jin-Der Wen and Donald M. Gray
Nucleic Acids Res. 2004; 32: e182. [Abstract] [Full text] [Print PDF]  

Methods Online
Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications
R. G. Rutledge
Nucleic Acids Res. 2004; 32: e178. [Abstract] [Full text] [Print PDF] [ Supplementary Material ] [ Supplementary Material ]  

Methods Online
Surface plasmon field-enhanced fluorescence spectroscopy in PCR product analysis by peptide nucleic acid probes
Danfeng Yao, Fang Yu, Junyoung Kim, Judith Scholz, Peter E. Nielsen, Eva-Kathrin Sinner, and Wolfgang Knoll
Nucleic Acids Res. 2004; 32: e177. [Abstract] [Full text] [Print PDF]  

Articles
Cloning of CviPII nicking and modification system from chlorella virus NYs-1 and application of Nt.CviPII in random DNA amplification
Siu-hong Chan, Zhenyu Zhu, James L. Van Etten, and Shuang-yong Xu
Nucleic Acids Res. 2004; 32: 6187-6199. [Abstract] [Full text] [Print PDF]  

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