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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 71-80 of 140 total displayed.

Past content

Methods Online
Protein-mediated error correction for de novo DNA synthesis
Peter A. Carr, Jason S. Park, Yoon-Jae Lee, Tiffany Yu, Shuguang Zhang, and Joseph M. Jacobson
Nucleic Acids Res. 2004; 32: e162. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
Microfluidic PicoArray synthesis of oligodeoxynucleotides and simultaneous assembling of multiple DNA sequences
Xiaochuan Zhou, Shiying Cai, Ailing Hong, Qimin You, Peilin Yu, Nijing Sheng, Onnop Srivannavit, Seema Muranjan, Jean Marie Rouillard, Yongmei Xia, Xiaolin Zhang, Qin Xiang, Renuka Ganesh, Qi Zhu, Anna Matejko, Erdogan Gulari, and Xiaolian Gao
Nucleic Acids Res. 2004; 32: 5409-5417. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR
Brooks E. Miner, Reinhard J. Stöger, Alice F. Burden, Charles D. Laird, and R. Scott Hansen
Nucleic Acids Res. 2004; 32: e135. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
Amplification and assembly of chip-eluted DNA (AACED): a method for high-throughput gene synthesis
Kathryn E. Richmond, Mo-Huang Li, Matthew J. Rodesch, Madhusudan Patel, Aaron M. Lowe, Changhan Kim, Larry L. Chu, Narasimhar Venkataramaian, Shane F. Flickinger, James Kaysen, Peter J. Belshaw, Michael R. Sussman, and Franco Cerrina
Nucleic Acids Res. 2004; 32: 5011-5018. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Use of pteridine nucleoside analogs as hybridization probes
Mary E. Hawkins and Frank M. Balis
Nucleic Acids Res. 2004; 32: e62. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Instant evaluation of the absolute initial number of cDNA copies from a single real-time PCR curve
Stéphane Swillens, Jean-Christophe Goffard, Yoann Maréchal, Alban de Kerchove d’Exaerde, and Hakim El Housni
Nucleic Acids Res. 2004; 32: e56. [Abstract] [Full text] [Print PDF]  

Articles
A third base pair for the polymerase chain reaction: inserting isoC and isoG
Scott C. Johnson, Christopher B. Sherrill, David J. Marshall, Michael J. Moser, and James R. Prudent
Nucleic Acids Res. 2004; 32: 1937-1941. [Abstract] [Full text] [Print PDF]  

Articles
PCR amplification of DNA containing non-standard base pairs by variants of reverse transcriptase from Human Immunodeficiency Virus-1
A. Michael Sismour, Stefan Lutz, Jeong-Ho Park, Michael J. Lutz, Paul L. Boyer, Stephen H. Hughes, and Steven A. Benner
Nucleic Acids Res. 2004; 32: 728-735. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Selective DNA amplification from complex genomes using universal double-sided adapters
Matthew J. Callow, Snezana Drmanac, and Radoje Drmanac
Nucleic Acids Res. 2004; 32: e21. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Global amplification of mRNA by template-switching PCR: linearity and application to microarray analysis
L. Petalidis, S. Bhattacharyya, G. A. Morris, V. P. Collins, T. C. Freeman, and P. A. Lyons
Nucleic Acids Res. 2003; 31: e142. [Abstract] [Full text] [Print PDF]  

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