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Nucleic acid amplification


This category presents in vivo and in vitro methods for generation of identical synthetic copies of RNA and DNA. These methods may operate at micro or macro scales and at various or uniform temperatures. The method may address a specific new strategy for nucleic acid amplification or a new application or significant enhancement of previously described protocols.


Citations 81-90 of 140 total displayed.

Past content

NAR Methods Online
Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences
Xinxin Gao, Peggy Yo, Andrew Keith, Timothy J. Ragan, and Thomas K. Harris
Nucleic Acids Res. 2003; 31: e143. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR
Milko B. Kermekchiev, Anatoly Tzekov, and Wayne M. Barnes
Nucleic Acids Res. 2003; 31: 6139-6147. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

NAR Methods Online
A novel real-time quantitative PCR method using attached universal template probe
Yuanli Zhang, Dabing Zhang, Wenquan Li, Jianqun Chen, Yufa Peng, and Wei Cao
Nucleic Acids Res. 2003; 31: e123. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Sequence-specific fluorescent labeling of double-stranded DNA observed at the single molecule level
Bénédicte Géron-Landre, Thibaut Roulon, Pierre Desbiolles, and Christophe Escudé
Nucleic Acids Res. 2003; 31: e125. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Ligation mediated PCR performed at low denaturation temperatures—PCR melting profiles
Aleksander Masny and Andrzej Plucienniczak
Nucleic Acids Res. 2003; 31: e114. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Kinetic Outlier Detection (KOD) in real-time PCR
Tzachi Bar, Anders Ståhlberg, Anders Muszta, and Mikael Kubista
Nucleic Acids Res. 2003; 31: e105. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

NAR Methods Online
Regionalized GC content of template DNA as a predictor of PCR success
Yair Benita, Ronald S. Oosting, Martin C. Lok, Michael J. Wise, and Ian Humphery-Smith
Nucleic Acids Res. 2003; 31: e99. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis
Stuart N. Peirson, Jason N. Butler, and Russell G. Foster
Nucleic Acids Res. 2003; 31: e73. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

NAR Methods Online
A novel multiplex quantitative DNA array based PCR (MQDA-PCR) for quantification of transgenic maize in food and feed
Knut Rudi, Ida Rud, and Askild Holck
Nucleic Acids Res. 2003; 31: e62. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A new minor groove binding asymmetric cyanine reporter dye for real-time PCR
Martin Bengtsson, H. Jonas Karlsson, Gunnar Westman, and Mikael Kubista
Nucleic Acids Res. 2003; 31: e45. [Abstract] [Full text] [Print PDF]  

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