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Nucleic acid modification


This category presents chemical and biophysical methods for identifying specific modifications as well as biological approaches for assaying functional modifications of DNA and RNA and their consequences in vivo.


Citations 11-20 of 94 total displayed.

Past content

Methods Online
Light activation of transcription: photocaging of nucleotides for control over RNA polymerization
André Vidal Pinheiro, Pedro Baptista, and João Carlos Lima
Nucleic Acids Res. 2008; 36: e90. [Abstract] [Full text] [Print PDF]  

Molecular Biology
DNA methylation analysis by digital bisulfite genomic sequencing and digital MethyLight
Daniel J. Weisenberger, Binh N. Trinh, Mihaela Campan, Shikhar Sharma, Tiffany I. Long, Suchitra Ananthnarayan, Gangning Liang, Francisco J. Esteva, Gabriel N. Hortobagyi, Frank McCormick, Peter A. Jones, and Peter W. Laird
Nucleic Acids Res. 2008; 36: 4689-4698. [Abstract] [Full text] [Print PDF]  

Methods Online
Inversing the natural hydrogen bonding rule to selectively amplify GC-rich ADAR-edited RNAs
Rodolphe Suspène, Myrtille Renard, Michel Henry, Denise Guétard, David Puyraimond-Zemmour, Agnès Billecocq, Michèle Bouloy, Frederic Tangy, Jean-Pierre Vartanian, and Simon Wain-Hobson
Nucleic Acids Res. 2008; 36: e72. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Nucleic Acid Enzymes
Real-time kinetics of restriction–modification gene expression after entry into a new host cell
Iwona Mruk and Robert M. Blumenthal
Nucleic Acids Res. 2008; 36: 2581-2593. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Bisulfite sequencing Data Presentation and Compilation (BDPC) web server—a useful tool for DNA methylation analysis
Christian Rohde, Yingying Zhang, Tomasz P. Jurkowski, Heinrich Stamerjohanns, Richard Reinhardt, and Albert Jeltsch
Nucleic Acids Res. 2008; 36: e34. [Abstract] [Full text] [Print PDF]  

Methods Online
A ligation assay for multiplex analysis of CpG methylation using bisulfite-treated DNA
Christina Dahl and Per Guldberg
Nucleic Acids Res. 2007; 35: e144. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Chemistry
Termination of DNA synthesis by N6-alkylated, not 3'-O-alkylated, photocleavable 2'-deoxyadenosine triphosphates
Weidong Wu, Brian P. Stupi, Vladislav A. Litosh, Dena Mansouri, Demetra Farley, Sidney Morris, Sherry Metzker, and Michael L. Metzker
Nucleic Acids Res. 2007; 35: 6339-6349. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons
Anne-Laure Chateigner-Boutin and Ian Small
Nucleic Acids Res. 2007; 35: e114. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Genomic profiling of CpG methylation and allelic specificity using quantitative high-throughput mass spectrometry: critical evaluation and improvements
Marcel W. Coolen, Aaron L. Statham, Margaret Gardiner-Garden, and Susan J. Clark
Nucleic Acids Res. 2007; 35: e119. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Selection of restriction endonucleases using artificial cells
Yu Zheng and Richard J. Roberts
Nucleic Acids Res. 2007; 35: e83. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

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