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Protein-protein interaction


This category describes methods using nucleic acids for the analysis of protein-protein interactions or methods specifically suited for the analysis of interactions between proteins involved in nucleic acid function. This includes, but is not limited to, two-hybrid system, surface display, arrays, generation of mutant libraries.


Citations 21-30 of 41 total displayed.

Past content

Methods Online
A dual-light reporter system to determine the efficiency of protein–protein interactions in mammalian cells
M. T. Nasim and R. C. Trembath
Nucleic Acids Res. 2005; 33: e66. [Abstract] [Full text] [Print PDF]  

Methods Online
Proximity extension of circular DNA aptamers with real-time protein detection
Daniel A. Di Giusto, Wjatschesslaw A. Wlassoff, J. Justin Gooding, Barbara A. Messerle, and Garry C. King
Nucleic Acids Res. 2005; 33: e64. [Abstract] [Full text] [Print PDF]  

Methods Online
Determination of tumor necrosis factor receptor-associated factor trimerization in living cells by CFP->YFP->mRFP FRET detected by flow cytometry
Liusheng He, Xiaoli Wu, James Simone, Derek Hewgill, and Peter E. Lipsky
Nucleic Acids Res. 2005; 33: e61. [Abstract] [Full text] [Print PDF]  

Methods Online
Selection of functional human antibodies from retroviral display libraries
Johannes H. Urban, Richard M. Schneider, Marta Compte, Carsten Finger, Klaus Cichutek, Luis Álvarez-Vallina, and Christian J. Buchholz
Nucleic Acids Res. 2005; 33: e35. [Abstract] [Full text] [Print PDF]  

Methods Online
Novel mobilizable prokaryotic two-hybrid system vectors for high-throughput protein interaction mapping in Escherichia coli by bacterial conjugation
Paul Clarke, Páraic Ó Cuív, and Michael O'Connell
Nucleic Acids Res. 2005; 33: e18. [Abstract] [Full text] [Print PDF]  

Articles
Protein interaction mapping on a functional shotgun sequence of Rickettsia sibirica
Joel A. Malek, Jamey M. Wierzbowski, Wei Tao, Stephanie A. Bosak, David J. Saranga, Lynn Doucette-Stamm, Douglas R. Smith, Paul J. McEwan, and Kevin J. McKernan
Nucleic Acids Res. 2004; 32: 1059-1064. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Articles
Exhaustive identification of interaction domains using a high-throughput method based on two-hybrid screening and PCR-convergence: molecular dissection of a kinetochore subunit Spc34p
Akinori Ikeuchi, Yoko Sasaki, Yasuaki Kawarasaki, and Tsuneo Yamane
Nucleic Acids Res. 2003; 31: 6953-6962. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Combination of DNA-directed immobilization and immuno-PCR: very sensitive antigen detection by means of self-assembled DNA–protein conjugates
Christof M. Niemeyer, Ron Wacker, and Michael Adler
Nucleic Acids Res. 2003; 31: e90. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Adaptation of the Ras-recruitment system to the analysis of interactions between membrane-associated proteins
Fabian Köhler and Kristian M. Müller
Nucleic Acids Res. 2003; 31: e28. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
De novo production of diverse intracellular antibody libraries
Tomoyuki Tanaka, Grace T. Y. Chung, Alan Forster, M. Natividad Lobato, and Terence H. Rabbitts
Nucleic Acids Res. 2003; 31: e23. [Abstract] [Full text] [Print PDF]  

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