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Recombinant DNA expression
This category describes methods for the expression of recombinant DNA in vitro or in various hosts. Expression of the DNA can be at the protein or RNA level and involved regulated or constitutive expression. An extension of these techniques may involve transgenic animals for ectopic expression, tissue specific expression, mutant rescue or involve gene therapy.
Citations 1-10 of 70 total displayed.
- Methods Online
Alternative-splicing-based bicistronic vectors for ratio-controlled protein expression and application to recombinant antibody production
- Stéphanie Fallot, Raouia Ben Naya, Corinne Hieblot, Philippe Mondon, Eric Lacazette, Khalil Bouayadi, Abdelhakim Kharrat, Christian Touriol, and Hervé Prats
Nucleic Acids Res. 2009; 37: e134.
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- Methods Online
A fast and efficient translational control system for conditional expression of yeast genes
- Peter Kötter, Julia E. Weigand, Britta Meyer, Karl-Dieter Entian, and Beatrix Suess
Nucleic Acids Res. 2009; 37: e120.
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- Methods Online
An efficient method to assemble linear DNA templates for in vitro screening and selection systems
- Viktor Stein and Florian Hollfelder
Nucleic Acids Res. 2009; 37: e122.
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- Methods Online
Expression-independent gene trap vectors for random and targeted mutagenesis in embryonic stem cells
- Anestis Tsakiridis, Elena Tzouanacou, Afifah Rahman, Douglas Colby, Richard Axton, Ian Chambers, Valerie Wilson, Lesley Forrester, and Joshua M. Brickman
Nucleic Acids Res. 2009; 37: e129.
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- Methods Online
cDNA display: a novel screening method for functional disulfide-rich peptides by solid-phase synthesis and stabilization of mRNA–protein fusions
- Junichi Yamaguchi, Mohammed Naimuddin, Manish Biyani, Toru Sasaki, Masayuki Machida, Tai Kubo, Takashi Funatsu, Yuzuru Husimi, and Naoto Nemoto
Nucleic Acids Res. 2009; 37: e108.
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- Methods Online
Studying human telomerase gene transcription by a chromatinized reporter generated by recombinase-mediated targeting of a bacterial artificial chromosome
- Shuwen Wang, Yuanjun Zhao, Melanie A. Leiby, and Jiyue Zhu
Nucleic Acids Res. 2009; 37: e111.
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- Methods Online
Rapid antibody selection by mRNA display on a microfluidic chip
- Noriko Tabata, Yuko Sakuma, Yumiko Honda, Nobuhide Doi, Hideaki Takashima, Etsuko Miyamoto-Sato, and Hiroshi Yanagawa
Nucleic Acids Res. 2009; 37: e64.
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- Methods Online
Selection of complementary single-variable domains for building monoclonal antibodies to native proteins
- Tomoyuki Tanaka and Terence H. Rabbitts
Nucleic Acids Res. 2009; 37: e41.
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- Methods Online
Manipulating transgenes using a chromosome vector
- Masashi Ikeno, Nobutaka Suzuki, Yoshinori Hasegawa, and Tsuneko Okazaki
Nucleic Acids Res. 2009; 37: e44.
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- Methods Online
Inducible expression of coding and inhibitory RNAs from retargetable genomic loci
- Ina Weidenfeld, Manfred Gossen, Rainer Löw, David Kentner, Stefan Berger, Dirk Görlich, Dusan Bartsch, Hermann Bujard, and Kai Schönig
Nucleic Acids Res. 2009; 37: e50.
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