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Recombinant DNA expression
This category describes methods for the expression of recombinant DNA in vitro or in various hosts. Expression of the DNA can be at the protein or RNA level and involved regulated or constitutive expression. An extension of these techniques may involve transgenic animals for ectopic expression, tissue specific expression, mutant rescue or involve gene therapy.
Citations 1-10 of 49 total displayed.
- Methods Online
The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a –70/+96 mRNA region with respect to the translation initiation codon
- S. Care, C. Bignon, M. C. Pelissier, E. Blanc, B. Canard, and B. Coutard
Nucleic Acids Res. 2008; 36: e6.
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- Methods Online
An engineered L-arginine sensor of Chlamydia pneumoniae enables arginine-adjustable transcription control in mammalian cells and mice
- Shizuka Hartenbach, Marie Daoud-El Baba, Wilfried Weber, and Martin Fussenegger
Nucleic Acids Res. 2007; 35: e136.
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- Methods Online
A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus
- Martin Thanbichler, Antonio A. Iniesta, and Lucy Shapiro
Nucleic Acids Res. 2007; 35: e137.
[Abstract]
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- Methods Online
An S/MAR-based infectious episomal genomic DNA expression vector provides long-term regulated functional complementation of LDLR deficiency
- Michele M.P. Lufino, Roberto Manservigi, and Richard Wade-Martins
Nucleic Acids Res. 2007; 35: e98.
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- Methods Online
Motif programming: a microgene-based method for creating synthetic proteins containing multiple functional motifs
- Hirohide Saito, Tamiko Minamisawa, and Kiyotaka Shiba
Nucleic Acids Res. 2007; 35: e38.
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- Methods Online
Vectors for co-expression of an unrestricted number of proteins
- Christoph Scheich, Daniel Kümmel, Dimitri Soumailakakis, Udo Heinemann, and Konrad Büssow
Nucleic Acids Res. 2007; 35: e43.
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- Methods Online
A synthetic luxCDABE gene cluster optimized for expression in high-GC bacteria
- Arryn Craney, Tobias Hohenauer, Ye Xu, Naveen Kumar Navani, Yingfu Li, and Justin Nodwell
Nucleic Acids Res. 2007; 35: e46.
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- Methods Online
Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli
- Nina Bandmann and Per-Åke Nygren
Nucleic Acids Res. 2007; 35: e32.
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- Methods Online
Eliminating helper phage from phage display
- L. Chasteen, J. Ayriss, P. Pavlik, and A. R. M. Bradbury
Nucleic Acids Res. 2006; 34: e145.
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- Methods Online
Troubleshooting coupled in vitro transcription–translation system derived from Escherichia coli cells: synthesis of high-yield fully active proteins
- Madina B. Iskakova, Witold Szaflarski, Marc Dreyfus, Jaanus Remme, and Knud H. Nierhaus
Nucleic Acids Res. 2006; 34: e135.
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