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Recombinant DNA expression
This category describes methods for the expression of recombinant DNA in vitro or in various hosts. Expression of the DNA can be at the protein or RNA level and involved regulated or constitutive expression. An extension of these techniques may involve transgenic animals for ectopic expression, tissue specific expression, mutant rescue or involve gene therapy.
Citations 11-20 of 70 total displayed.
- Methods Online
Experimental analysis of gene assembly with TopDown one-step real-time gene synthesis
- Hongye Ye, Mo Chao Huang, Mo-Huang Li, and Jackie Y. Ying
Nucleic Acids Res. 2009; 37: e51.
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- Methods Online
Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells
- Omar Nyabi, Michael Naessens, Katharina Haigh, Agnieszka Gembarska, Steven Goossens, Marion Maetens, Sarah De Clercq, Benjamin Drogat, Lieven Haenebalcke, Sonia Bartunkova, Ilse De Vos, Bram De Craene, Mansour Karimi, Geert Berx, Andras Nagy, Pierre Hilson, Jean-Christophe Marine, and Jody J. Haigh
Nucleic Acids Res. 2009; 37: e55.
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- Methods Online
Epitope tagging of endogenous genes in diverse human cell lines
- Jung-Sik Kim, Challice Bonifant, Fred Bunz, William S. Lane, and Todd Waldman
Nucleic Acids Res. 2008; 36: e127.
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- Methods Online
Homogeneity and persistence of transgene expression by omitting antibiotic selection in cell line isolation
- Weimin L. Kaufman, Ibrahim Kocman, Vishal Agrawal, Hans-Peter Rahn, Daniel Besser, and Manfred Gossen
Nucleic Acids Res. 2008; 36: e111.
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- Methods Online
A functional selection of viral genetic elements in cultured cells to identify hepatitis C virus RNA translation inhibitors
- Loic Jaffrelo, Sandrine Chabas, Sandrine Reigadas, Aude Pflieger, Czeslaw Wychowski, Julie Rumi, Michel Ventura, Jean-Jacques Toulmé, and Cathy Staedel
Nucleic Acids Res. 2008; 36: e95.
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- Methods Online
Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions
- Gaurav Backliwal, Markus Hildinger, Sebastien Chenuet, Sarah Wulhfard, Maria De Jesus, and Florian M. Wurm
Nucleic Acids Res. 2008; 36: e96.
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- Methods Online
Intronically encoded siRNAs improve dynamic range of mammalian gene regulation systems and toggle switch
- David Greber, Marie Daoud El-Baba, and Martin Fussenegger
Nucleic Acids Res. 2008; 36: e101.
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- Methods Online
Linking the functions of unrelated proteins using a novel directed evolution domain insertion method
- Wayne R. Edwards, Kathy Busse, Rudolf K. Allemann, and D. Dafydd Jones
Nucleic Acids Res. 2008; 36: e78.
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- Structural Biology
Structural analysis of the genetic switch that regulates the expression of restriction-modification genes
- John E. McGeehan, Simon D. Streeter, Sarah -J. Thresh, Neil Ball, Raimond B. G. Ravelli, and G. Geoff Kneale
Nucleic Acids Res. 2008; 36: 4778-4787.
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- Methods Online
Nuclear delivery of NF B-assisted DNA/polymer complexes: plasmid DNA quantitation by confocal laser scanning microscopy and evidence of nuclear polyplexes by FRET imaging
- Gilles Breuzard, Magdalena Tertil, Cristine Gonçalves, Hervé Cheradame, Philippe Géguan, Chantal Pichon, and Patrick Midoux
Nucleic Acids Res. 2008; 36: e71.
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