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Recombinant DNA expression
This category describes methods for the expression of recombinant DNA in vitro or in various hosts. Expression of the DNA can be at the protein or RNA level and involved regulated or constitutive expression. An extension of these techniques may involve transgenic animals for ectopic expression, tissue specific expression, mutant rescue or involve gene therapy.
Citations 31-40 of 70 total displayed.
- Methods Online
Troubleshooting coupled in vitro transcription–translation system derived from Escherichia coli cells: synthesis of high-yield fully active proteins
- Madina B. Iskakova, Witold Szaflarski, Marc Dreyfus, Jaanus Remme, and Knud H. Nierhaus
Nucleic Acids Res. 2006; 34: e135.
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- Methods Online
Fluorescent T7 display phages obtained by translational frameshift
- Erik J. Slootweg, Hans J.H.G. Keller, Mark A. Hink, Jan Willem Borst, Jaap Bakker, and Arjen Schots
Nucleic Acids Res. 2006; 34: e137.
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- Methods Online
A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus
- Tiziana Aucelli, Patrizia Contursi, Michele Girfoglio, Mosè Rossi, and Raffaele Cannio
Nucleic Acids Res. 2006; 34: e114.
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- Methods Online
Inducible microRNA expression by an all-in-one episomal vector system
- Alexey Epanchintsev, Peter Jung, Antje Menssen, and Heiko Hermeking
Nucleic Acids Res. 2006; 34: e119.
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- Methods Online
A spreadable, non-integrative and high copy number shuttle vector for Sulfolobus solfataricus based on the genetic element pSSVx from Sulfolobus islandicus
- Tiziana Aucelli, Patrizia Contursi, Michele Girfoglio, Mosè Rossi, and Raffaele Cannio
Nucleic Acids Res. Advance Access published on September 13, 2006; doi: 10.1093/nar/gkl615.
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- Methods Online
A new generation of vectors with increased induction ratios by overimposing a second regulatory level by attenuation
- Jose Luis Royo, Hamid Manyani, Angel Cebolla, and Eduardo Santero
Nucleic Acids Res. 2005; 33: e169.
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- Methods Online
Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange
- Ee Tsin Wong, John L Kolman, Yao-Cheng Li, Larry D Mesner, Wolfgang Hillen, Christian Berens, and Geoffrey M Wahl
Nucleic Acids Res. 2005; 33: e147.
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- Methods Online
Stringent doxycycline-dependent control of gene activities using an episomal one-vector system
- Georg W. Bornkamm, Christian Berens, Conny Kuklik-Roos, Jean-Marie Bechet, Gerhard Laux, Jürgen Bachl, Martin Korndoerfer, Martin Schlee, Michael Hölzel, Anastassia Malamoussi, Rob D. Chapman, Falk Nimmerjahn, Josef Mautner, Wolfgang Hillen, Hermann Bujard, and Jean Feuillard
Nucleic Acids Res. 2005; 33: e137.
[Abstract]
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- Articles
Heterologous gene expression using self-assembled supra-molecules with high affinity for HSP70 chaperone
- Ji-Young Ahn, Hyung Choi, Yang-Hoon Kim, Kyung-Yeon Han, Jin-Seung Park, Sung-Sik Han, and Jeewon Lee
Nucleic Acids Res. 2005; 33: 3751-3762.
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- Methods Online
A novel mammalian expression system derived from components coordinating nicotine degradation in arthrobacter nicotinovorans pAO1
- Laetitia Malphettes, Cornelia C. Weber, Marie Daoud El-Baba, Ronald G. Schoenmakers, Dominique Aubel, Wilfried Weber, and Martin Fussenegger
Nucleic Acids Res. 2005; 33: e107.
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