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Recombinant DNA expression
This category describes methods for the expression of recombinant DNA in vitro or in various hosts. Expression of the DNA can be at the protein or RNA level and involved regulated or constitutive expression. An extension of these techniques may involve transgenic animals for ectopic expression, tissue specific expression, mutant rescue or involve gene therapy.
Citations 41-50 of 70 total displayed.
- Methods Online
Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces
- Antonio Rodríguez-García, Patricia Combes, Rosario Pérez-Redondo, Matthew C. A. Smith, and Margaret C. M. Smith
Nucleic Acids Res. 2005; 33: e87.
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- Methods Online
A novel transgenic chimaeric mouse system for the rapid functional evaluation of genes encoding secreted proteins
- Makoto Kakitani, Takeshi Oshima, Kaori Horikoshi, Tetsuo Yoshitome, Akiko Ueda, Miwa Kajikawa, Yumi Iba, Yoshinao Ozone, Yuki Ijima, Tohko Yoshino, Mikiko Itoh, Sachiko Seki, Ayako Aoki, Toshie Ishihara, Michiyo Shionoya, Utako Makino, Rina Kitada, Atsuko Ohguma, Takami Ohta, Yoshimasa Yoshida, Hiroe Kudoh, Kazunori Hanaoka, Kazunori Sibuya, Isao Ishida, Minoru Kakeda, Mikio Yagi, Takashi Yoneya, and Kazuma Tomizuka
Nucleic Acids Res. 2005; 33: e85.
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- Methods Online
Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter
- David Markusic, Ronald Oude-Elferink, Atze T. Das, Ben Berkhout, and Jurgen Seppen
Nucleic Acids Res. 2005; 33: e63.
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- Methods Online
Screening of retroviral cDNA libraries for factors involved in protein phosphorylation in signaling cascades
- J. Stitz, P. O. Krutzik, and G. P. Nolan
Nucleic Acids Res. 2005; 33: e39.
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- Articles
DNA recombination with a heterospecific Cre homolog identified from comparison of the pac-c1 regions of P1-related phages
- Brian Sauer and Jeffrey McDermott
Nucleic Acids Res. 2004; 32: 6086-6095.
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- Methods Online
Design and in vivo characterization of self-inactivating human and non-human lentiviral expression vectors engineered for streptogramin-adjustable transgene expression
- Barbara Mitta, Cornelia C. Weber, Markus Rimann, and Martin Fussenegger
Nucleic Acids Res. 2004; 32: e106.
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- NAR Methods Online
RNA stem–loop enhanced expression of previously non-expressible genes
- Michael Paulus, Martin Haslbeck, and Manfred Watzele
Nucleic Acids Res. 2004; 32: e78.
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- NAR Methods Online
Artificial genetic selection for an efficient translation initiation site for expression of human RACK1 gene in Escherichia coli
- Olga B. Zhelyabovskaya, Yuri A. Berlin, and Klara R. Birikh
Nucleic Acids Res. 2004; 32: e52.
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- NAR Methods Online
Dual-regulated expression of C/EBP- and BMP-2 enables differential differentiation of C2C12 cells into adipocytes and osteoblasts
- Cornelia Fux, Barbara Mitta, Beat P. Kramer, and Martin Fussenegger
Nucleic Acids Res. 2004; 32: e1.
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- NAR Methods Online
Exploiting features of adenovirus replication to support mammalian kinase production
- Matt Cotten, Kerstin Stegmueller, Jan Eickhoff, Miriam Hanke, Katrin Herzberger, Thomas Herget, Axel Choidas, Henrik Daub, and Klaus Godl
Nucleic Acids Res. 2003; 31: e128.
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