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Recombinant DNA expression


This category describes methods for the expression of recombinant DNA in vitro or in various hosts. Expression of the DNA can be at the protein or RNA level and involved regulated or constitutive expression. An extension of these techniques may involve transgenic animals for ectopic expression, tissue specific expression, mutant rescue or involve gene therapy.

Citations 51-60 of 70 total displayed.

Past content

NAR Methods Online
Conditional human VEGF-mediated vascularization in chicken embryos using a novel temperature-inducible gene regulation (TIGR) system
Wilfried Weber, René R. Marty, Nils Link, Martin Ehrbar, Bettina Keller, Cornelia C. Weber, Andreas H. Zisch, Christoph Heinzen, Valentin Djonov, and Martin Fussenegger
Nucleic Acids Res. 2003; 31: e69. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
A novel helper phage that improves phage display selection efficiency by preventing the amplification of phages without recombinant protein
R. Arjen Kramer, Freek Cox, Marieke van der Horst, Sonja van den Oudenrijn, Pieter C. M. Res, Judith Bia, Ton Logtenberg, and John de Kruif
Nucleic Acids Res. 2003; 31: e59. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Novel retroviral vectors to facilitate expression screens in mammalian cells
Eugene Y. Koh, Tong Chen, and George Q. Daley
Nucleic Acids Res. 2002; 30: e142. [Abstract] [Full text] [Print PDF]  

Articles
Intein-mediated purification of cytotoxic endonuclease I-TevI by insertional inactivation and pH-controllable splicing
Wei Wu, David W. Wood, Georges Belfort, Victoria Derbyshire, and Marlene Belfort
Nucleic Acids Res. 2002; 30: 4864-4871. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
An improved helper phage system for efficient isolation of specific antibody molecules in phage display
Hyunjung Baek, Kyoung-ho Suk, Yong-hwan Kim, and Sanghoon Cha
Nucleic Acids Res. 2002; 30: e18. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells
Yves Durocher, Sylvie Perret, and Amine Kamen
Nucleic Acids Res. 2002; 30: e9. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Expression and purification of recombinant human c-Fos/c-Jun that is highly active in DNA binding and transcriptional activation in vitro
Heather A. Ferguson and James A. Goodrich
Nucleic Acids Res. 2001; 29: e98. [Abstract] [Full text] [Print PDF]  

Articles
Gene expression in isolated plant mitochondria: high fidelity of transcription, splicing and editing of a transgene product in electroporated organelles
Jean-Claude Farré and Alejandro Araya
Nucleic Acids Res. 2001; 29: 2484-2491. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
The rational design of a ‘type 88’ genetically stable peptide display vector in the filamentous bacteriophage fd
David Enshell-Seijffers, Larisa Smelyanski, and Jonathan M. Gershoni
Nucleic Acids Res. 2001; 29: e50. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Rapid generation of incremental truncation libraries for protein engineering using {alpha}-phosphothioate nucleotides
Stefan Lutz, Marc Ostermeier, and Stephen J. Benkovic
Nucleic Acids Res. 2001; 29: e16. [Abstract] [Full text] [Print PDF]  

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