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Recombinant DNA expression


This category describes methods for the expression of recombinant DNA in vitro or in various hosts. Expression of the DNA can be at the protein or RNA level and involved regulated or constitutive expression. An extension of these techniques may involve transgenic animals for ectopic expression, tissue specific expression, mutant rescue or involve gene therapy.


Citations 61-70 of 70 total displayed.

Past content

NAR Methods Online
A novel tetracycline-dependent transactivator with E2F4 transcriptional activation domain
Kiwamu Akagi, Masayuki Kanai, Hideyuki Saya, Tomoko Kozu, and Anton Berns
Nucleic Acids Res. 2001; 29: e23. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Inducible expression of exogenous genes in Dictyostelium discoideum using the ribonucleotide reductase promoter
Pascale Gaudet, Harry MacWilliams, and Adrian Tsang
Nucleic Acids Res. 2001; 29: e5. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Inducible and irreversible control of gene expression using a single transgene
Edya Fuhrmann-Benzakein, Irène García-Gabay, Michael S. Pepper, Jean-Dominique Vassalli, and Pedro Luis Herrera
Nucleic Acids Res. 2000; 28: e99. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
An extrachromosomal tetracycline-regulatable system for mammalian cells
Christopher R. Sclimenti, Edward J. Baba, and Michele P. Calos
Nucleic Acids Res. 2000; 28: e80. [Abstract] [Full text] [Print PDF]  

Articles
Pre-selection of integration sites imparts repeatable transgene expression
Helen Wallace, Ray Ansell, John Clark, and Jim McWhir
Nucleic Acids Res. 2000; 28: 1455-1464. [Abstract] [Full text] [Print PDF]  

NAR Methods Online
Gene targeting restricted to mouse striated muscle lineage
P Miniou, D Tiziano, T Frugier, N Roblot, M Le Meur, and J Melki
Nucleic Acids Res. 1999; 27: e27. [Abstract] [Full text] [Print PDF]  

Articles
Efficient bicistronic expression of cre in mammalian cells
JA Gorski and KR Jones
Nucleic Acids Res. 1999; 27: 2059-2061. [Abstract] [Full text] [Print PDF]  

Articles
Use of an internal ribosome entry site for bicistronic expression of Cre recombinase or rtTA transactivator
J Lindeberg and T Ebendal
Nucleic Acids Res. 1999; 27: 1552-1554. [Abstract] [Full text] [Print PDF]  

Articles
Vaccine candidate MSP-1 from Plasmodium falciparum: a redesigned 4917 bp polynucleotide enables synthesis and isolation of full-length protein from Escherichia coli and mammalian cells
W Pan, E Ravot, R Tolle, R Frank, R Mosbach, I Turbachova, and H Bujard
Nucleic Acids Res. 1999; 27: 1094-1103. [Abstract] [Full text] [Print PDF]  

Articles
Tetracycline-inducible expression systems with reduced basal activity in mammalian cells
K Forster, V Helbl, T Lederer, S Urlinger, N Wittenburg, and W Hillen
Nucleic Acids Res. 1999; 27: 708-710. [Abstract] [Full text] [Print PDF]  

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