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Recombination


This category describes methods making use of homologous, site specific or random recombination including their use in mutagenesis.


Citations 1-10 of 98 total displayed.

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Methods Online
Studying human telomerase gene transcription by a chromatinized reporter generated by recombinase-mediated targeting of a bacterial artificial chromosome
Shuwen Wang, Yuanjun Zhao, Melanie A. Leiby, and Jiyue Zhu
Nucleic Acids Res. 2009; 37: e111. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Past content

Methods Online
Restriction enzyme-free mutagenesis via the light regulation of DNA polymerization
Douglas D. Young, Hrvoje Lusic, Mark O. Lively, and Alexander Deiters
Nucleic Acids Res. 2009; 37: e58. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
In vivo evaluation of PhiC31 recombinase activity using a self-excision cassette
Eugenio Sangiorgi, Zhang Shuhua, and Mario R. Capecchi
Nucleic Acids Res. 2008; 36: e134. [Abstract] [Full text] [Print PDF]  

Methods Online
Efficient transfer of two large secondary metabolite pathway gene clusters into heterologous hosts by transposition
Jun Fu, Silke C. Wenzel, Olena Perlova, Junping Wang, Frank Gross, Zhiru Tang, Yulong Yin, A. Francis Stewart, Rolf Müller, and Youming Zhang
Nucleic Acids Res. 2008; 36: e113. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Recombineering-mediated tagging of Drosophila genomic constructs for in vivo localization and acute protein inactivation
Koen J. T. Venken, Jaroslaw Kasprowicz, Sabine Kuenen, Jiekun Yan, Bassem A. Hassan, and Patrik Verstreken
Nucleic Acids Res. 2008; 36: e114. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Advantages of q-PCR as a method of screening for gene targeting in mammalian cells using conventional and whole BAC-based constructs
Julio Gómez-Rodríguez, Valance Washington, Jun Cheng, Amalia Dutra, Evgenia Pak, Pentao Liu, Daniel W. McVicar, and Pamela L. Schwartzberg
Nucleic Acids Res. 2008; 36: e117. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Linking the functions of unrelated proteins using a novel directed evolution domain insertion method
Wayne R. Edwards, Kathy Busse, Rudolf K. Allemann, and D. Dafydd Jones
Nucleic Acids Res. 2008; 36: e78. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Rapid and efficient construction of markerless deletions in the Escherichia coli genome
Byung Jo Yu, Kui Hyeon Kang, Jun Hyoung Lee, Bong Hyun Sung, Mi Sun Kim, and Sun Chang Kim
Nucleic Acids Res. 2008; 36: e84. [Abstract] [Full text] [Print PDF]  

Methods Online
DNA bar coding and pyrosequencing to analyze adverse events in therapeutic gene transfer
Gary P. Wang, Alexandrine Garrigue, Angela Ciuffi, Keshet Ronen, Jeremy Leipzig, Charles Berry, Chantal Lagresle-Peyrou, Fatine Benjelloun, Salima Hacein-Bey-Abina, Alain Fischer, Marina Cavazzana-Calvo, and Frederic D. Bushman
Nucleic Acids Res. 2008; 36: e49. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli
Joseph A. DeVito
Nucleic Acids Res. 2008; 36: e4. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

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