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Recombination


This category describes methods making use of homologous, site specific or random recombination including their use in mutagenesis.

Citations 11-20 of 98 total displayed.

Past content

Methods Online
Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage {phi}BT1
Zhengyao Xu, Nicholas C. O. Lee, Felix Dafhnis-Calas, Sunir Malla, Margaret C. M. Smith, and William R. A. Brown
Nucleic Acids Res. 2008; 36: e9. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
In vivo construction of recombinant molecules within the Caenorhabditis elegans germ line using short regions of terminal homology
Benedict J. Kemp, Julia Hatzold, Laura A. Sternick, Joshua Cornman-Homonoff, Jessica M. Whitaker, Pamela J. Tieu, and Eric J. Lambie
Nucleic Acids Res. 2007; 35: e133. [Abstract] [Full text] [Print PDF]  

Molecular Biology
Identification of eukaryotic promoter regulatory elements using nonhomologous random recombination
Jeffrey B. Doyon and David R. Liu
Nucleic Acids Res. 2007; 35: 5851-5860. [Abstract] [Full text] [Print PDF]  

Methods Online
Assisted large fragment insertion by Red/ET-recombination (ALFIRE)—an alternative and enhanced method for large fragment recombineering
Adolfo Rivero-Müller, Svetlana Lajic, and Ilpo Huhtaniemi
Nucleic Acids Res. 2007; 35: e78. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Site-directed transposon integration in human cells
Stephen R. Yant, Yong Huang, Bassel Akache, and Mark A. Kay
Nucleic Acids Res. 2007; 35: e50. [Abstract] [Full text] [Print PDF]  

Molecular Biology
Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51
S. Desfarges, J. San Filippo, M. Fournier, C. Calmels, A. Caumont-Sarcos, S. Litvak, P. Sung, and V. Parissi
Nucleic Acids Res. 2006; 34: 6215-6224. [Abstract] [Full text] [Print PDF]  

Methods Online
A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences
Julianne Smith, Sylvestre Grizot, Sylvain Arnould, Aymeric Duclert, Jean-Charles Epinat, Patrick Chames, Jesús Prieto, Pilar Redondo, Francisco J. Blanco, Jerónimo Bravo, Guillermo Montoya, Frédéric Pâques, and Philippe Duchateau
Nucleic Acids Res. 2006; 34: e149. [Abstract] [Full text] [Print PDF]  

Methods Online
Genomic analysis of insertion behavior and target specificity of mini-Tn7 and Tn3 transposons in Saccharomyces cerevisiae
Michael Seringhaus, Anuj Kumar, John Hartigan, Michael Snyder, and Mark Gerstein
Nucleic Acids Res. 2006; 34: e57. [Abstract] [Full text] [Print PDF]  

Methods Online
Development and application of a positive–negative selectable marker system for use in reverse genetics in Plasmodium
Joanna A. M. Braks, Blandine Franke-Fayard, Hans Kroeze, Chris J. Janse, and Andrew P. Waters
Nucleic Acids Res. 2006; 34: e39. [Abstract] [Full text] [Print PDF]  

Methods Online
Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
Gholam Khodakaramian, Sarah Lissenden, Bertolt Gust, Laura Moir, Paul A. Hoskisson, Keith F. Chater, and Margaret C. M. Smith
Nucleic Acids Res. 2006; 34: e20. [Abstract] [Full text] [Print PDF]  

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