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Ribosomes and Protein Translation
This category presents methods for analyzing protein translational machinery in vivo and in vitro. Focus would include issues involving ribosomes, rRNA, tRNA, translation, protein synthesis, initiation factors, elongation factors, termination factors, and nucleoli. New and improved methods for protein translation or component purification will also be considered.
Citations 11-19 of 19 total displayed.
- Methods Online
Recombineering with tolC as a Selectable/Counter-selectable Marker: remodeling the rRNA Operons of Escherichia coli
- Joseph A. DeVito
Nucleic Acids Res. 2008; 36: e4.
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- Methods Online
The translation of recombinant proteins in E. coli can be improved by in silico generating and screening random libraries of a –70/+96 mRNA region with respect to the translation initiation codon
- S. Care, C. Bignon, M. C. Pelissier, E. Blanc, B. Canard, and B. Coutard
Nucleic Acids Res. 2008; 36: e6.
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- Methods Online
Back to basics: the untreated rabbit reticulocyte lysate as a competitive system to recapitulate cap/poly(A) synergy and the selective advantage of IRES-driven translation
- Ricardo Soto Rifo, Emiliano P. Ricci, Didier Décimo, Olivier Moncorgé, and Théophile Ohlmann
Nucleic Acids Res. 2007; 35: e121.
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- Methods Online
Use of signal sequences as an in situ removable sequence element to stimulate protein synthesis in cell-free extracts
- Jin-Ho Ahn, Mi-Yeon Hwang, Kyung-Ho Lee, Cha-Yong Choi, and Dong-Myung Kim
Nucleic Acids Res. 2007; 35: e21.
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- Methods Online
Troubleshooting coupled in vitro transcription–translation system derived from Escherichia coli cells: synthesis of high-yield fully active proteins
- Madina B. Iskakova, Witold Szaflarski, Marc Dreyfus, Jaanus Remme, and Knud H. Nierhaus
Nucleic Acids Res. 2006; 34: e135.
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- Methods Online
Fluorescent T7 display phages obtained by translational frameshift
- Erik J. Slootweg, Hans J.H.G. Keller, Mark A. Hink, Jan Willem Borst, Jaap Bakker, and Arjen Schots
Nucleic Acids Res. 2006; 34: e137.
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- Methods Online
Solid-phase translation and RNA–protein fusion: a novel approach for folding quality control and direct immobilization of proteins using anchored mRNA
- Manish Biyani, Yuzuru Husimi, and Naoto Nemoto
Nucleic Acids Res. 2006; 34: e140.
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- Methods Online
In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system
- Hikaru Taira, Takahiro Hohsaka, and Masahiko Sisido
Nucleic Acids Res. 2006; 34: e44.
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- Methods Online
A translation control reporter system (TCRS) for the analysis of translationally controlled processes in the vertebrate cell
- Volker Wiesenthal, Achim Leutz, and Cornelis F. Calkhoven
Nucleic Acids Res. 2006; 34: e23.
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