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RNA characterisation and manipulation


This category presents all methods to analyze RNA including, but not limited to, RNA production, enzymatic treatment of RNA, RNA sequencing and probing, strategies involving ribozymes, biophysical methods for the characterization of RNA.


Citations 1-10 of 141 total displayed.

Most recent content

Methods Online
In vivo expression and purification of aptamer-tagged small RNA regulators
Nelly Said, Renate Rieder, Robert Hurwitz, Jochen Deckert, Henning Urlaub, and Jörg Vogel
Nucleic Acids Res. 2009; 37: e133. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A high throughput experimental approach to identify miRNA targets in human cells
Lu Ping Tan, Erwin Seinen, Gerben Duns, Debora de Jong, Ody C. M. Sibon, Sibrand Poppema, Bart-Jan Kroesen, Klaas Kok, and Anke van den Berg
Nucleic Acids Res. 2009; 37: e137. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Past content

Methods Online
Transcriptome analysis by strand-specific sequencing of complementary DNA
Dmitri Parkhomchuk, Tatiana Borodina, Vyacheslav Amstislavskiy, Maria Banaru, Linda Hallen, Sylvia Krobitsch, Hans Lehrach, and Alexey Soldatov
Nucleic Acids Res. 2009; 37: e123. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Analysis of splicing patterns by pyrosequencing
Agnès Méreau, Vincent Anquetil, Marie Cibois, Maud Noiret, Aline Primot, Audrey Vallée, and Luc Paillard
Nucleic Acids Res. 2009; 37: e126. [Abstract] [Full text] [Print PDF]  

Methods Online
Conserved principles of mammalian transcriptional regulation revealed by RNA half-life
Caroline C. Friedel, Lars Dölken, Zsolt Ruzsics, Ulrich H. Koszinowski, and Ralf Zimmer
Nucleic Acids Res. 2009; 37: e115. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Ana M. Blanco, Laura Rausell, Begoña Aguado, Manuel Perez-Alonso, and Rubén Artero
Nucleic Acids Res. 2009; 37: e116. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Computational Biology
Stochastic noise in splicing machinery
Eugene Melamud and John Moult
Nucleic Acids Res. 2009; 37: 4873-4886. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Computational Biology
Accurate prediction of NAGNAG alternative splicing
Rileen Sinha, Swetlana Nikolajewa, Karol Szafranski, Michael Hiller, Niels Jahn, Klaus Huse, Matthias Platzer, and Rolf Backofen
Nucleic Acids Res. 2009; 37: 3569-3579. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

RNA
Constructing RNA dynamical ensembles by combining MD and motionally decoupled NMR RDCs: new insights into RNA dynamics and adaptive ligand recognition
Aaron T. Frank, Andrew C. Stelzer, Hashim M. Al-Hashimi, and Ioan Andricioaei
Nucleic Acids Res. 2009; 37: 3670-3679. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

Methods Online
Light-up properties of complexes between thiazole orange-small molecule conjugates and aptamers
Renjun Pei, Jeffrey Rothman, Yuli Xie, and Milan N. Stojanovic
Nucleic Acids Res. 2009; 37: e59. [Abstract] [Full text] [Print PDF] [ Supplementary Data ]  

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