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RNA characterisation and manipulation


This category presents all methods to analyze RNA including, but not limited to, RNA production, enzymatic treatment of RNA, RNA sequencing and probing, strategies involving ribozymes, biophysical methods for the characterization of RNA.


Citations 71-80 of 143 total displayed.

Past content

Methods Online
Development of an automated in vitro selection protocol to obtain RNA-based aptamers: identification of a biostable substance P antagonist
Dirk Eulberg, Klaus Buchner, Christian Maasch, and Sven Klussmann
Nucleic Acids Res. 2005; 33: e45. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Targeted ‘knockdown’ of spliceosome function in mammalian cells
Nathalie Matter and Harald König
Nucleic Acids Res. 2005; 33: e41. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Novel cyanine-AMP conjugates for efficient 5' RNA fluorescent labeling by one-step transcription and replacement of [{gamma}-32P]ATP in RNA structural investigation
Na Li, Changjun Yu, and Faqing Huang
Nucleic Acids Res. 2005; 33: e37. [Abstract] [Full text] [Print PDF]  

Methods Online
Development of a micro-array to detect human and mouse microRNAs and characterization of expression in human organs
Yingqing Sun, Seongjoon Koo, Neill White, Eigen Peralta, Christine Esau, Nicholas M. Dean, and Ranjan J. Perera
Nucleic Acids Res. 2004; 32: e188. [Abstract] [Full text] [Print PDF] [ Supplementary Material ]  

Methods Online
Tris-borate is a poor counterion for RNA: a cautionary tale for RNA folding studies
Karen L. Buchmueller and Kevin M. Weeks
Nucleic Acids Res. 2004; 32: e184. [Abstract] [Full text] [Print PDF]  

Methods Online
Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes
Anna Válóczi, Csaba Hornyik, Nóra Varga, József Burgyán, Sakari Kauppinen, and Zoltán Havelda
Nucleic Acids Res. 2004; 32: e175. [Abstract] [Full text] [Print PDF]  

Articles
RNA interference using boranophosphate siRNAs: structure–activity relationships
Allison H. S. Hall, Jing Wan, Erin E. Shaughnessy, Barbara Ramsay Shaw, and Kenneth A. Alexander
Nucleic Acids Res. 2004; 32: 5991-6000. [Abstract] [Full text] [Print PDF]  

Methods Online
Joining of long double-stranded RNA molecules through controlled overhangs
N. H. Dekker, J. A. Abels, P. T. M. Veenhuizen, M. M. Bruinink, and C. Dekker
Nucleic Acids Res. 2004; 32: e140. [Abstract] [Full text] [Print PDF]  

Articles
Exploration of the conserved A+C wobble pair within the ribosomal peptidyl transferase center using affinity purified mutant ribosomes
Ashley Eversole Hesslein, Vladimir I. Katunin, Malte Beringer, Anne B. Kosek, Marina V. Rodnina, and Scott A. Strobel
Nucleic Acids Res. 2004; 32: 3760-3770. [Abstract] [Full text] [Print PDF]  

Methods Online
Reverse Sanger sequencing of RNA by MALDI-TOF mass spectrometry after solid phase purification
Beatrice Spottke, Julia Gross, Hans-Joachim Galla, and Franz Hillenkamp
Nucleic Acids Res. 2004; 32: e97. [Abstract] [Full text] [Print PDF]  

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